PMID- 1855387
OWN - NLM
STAT- MEDLINE
DCOM- 19910829
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 57
IP  - 1
DP  - 1991
TI  - A fluorescence in situ hybridization technique for retrospective cytogenetic 
      analysis.
PG  - 16-7
AB  - Fluorescence in situ hybridization (FISH) is being used increasingly in clinical 
      practice; however, current FISH techniques require fresh material, and there is 
      considerable variation in hybridization efficiency between laboratories. We have 
      modified a FISH technique described by Pinkel et al. (1986) that works not only 
      on freshly G-banded material but also on cytogenetic preparations ranging in age 
      from 2 wk to 12 yr. We have tested this technique on several centromeric alphoid 
      satellite probes (D1Z5, D7Z1, D17Z1, DXZ1, and DYZ3) and one noncentromeric 
      minisatellite probe (D1Z2). Our average hybridization efficiency on freshly 
      banded preparations for these probes is consistently greater than 90%. The 
      combination of higher efficiency and the ability to perform hybridization on 
      previously G-banded material makes this a valuable technique for retrospective 
      analyses.
FAU - Babu, V R
AU  - Babu VR
AD  - Medical Genetics and Birth Defects Center, Henry Ford Hospital, Detroit, MI.
FAU - Wiktor, A
AU  - Wiktor A
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Child
MH  - Chromosome Banding
MH  - DNA Probes
MH  - DNA, Satellite/genetics
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Male
MH  - *Nucleic Acid Hybridization
MH  - Retrospective Studies
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1159/000133104 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1991;57(1):16-7. doi: 10.1159/000133104.