PMID- 18554159
OWN - NLM
STAT- MEDLINE
DCOM- 20080916
LR  - 20211020
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 27
IP  - 9
DP  - 2008 Sep
TI  - Soluble epoxide hydrolase homologs in Strongylocentrotus purpuratus suggest a 
      gene duplication event and subsequent divergence.
PG  - 467-77
LID - 10.1089/dna.2008.0751 [doi]
AB  - The mammalian soluble epoxide hydrolase (sEH) is a multidomain enzyme composed of 
      C- and N-terminal regions that contain active sites for epoxide hydrolase (EH) 
      and phosphatase activities, respectively. We report the cloning of two 60 kDa 
      multidomain enzymes from the purple sea urchin Strongylocentrotus purpuratus 
      displaying significant sequence similarity to both the N- and C-terminal domains 
      of the mammalian sEH. While one urchin enzyme did not exhibit EH activity, the 
      second enzyme hydrolyzed several lipid messenger molecules metabolized by the 
      mammalian sEH, including the epoxyeicosatrienoic acids. Neither of the urchin 
      enzymes displayed phosphatase activity. The urchin EH was inhibited by small 
      molecule inhibitors of the mammalian sEH and is the likely ancestor of the 
      enzyme. Sequence comparisons suggest that the urchin sEH homologs are the result 
      of a gene fusion event between a gene encoding for an EH and a gene for an enzyme 
      of undetermined function. This fusion event was followed by a duplication event 
      to produce the urchin enzymes.
FAU - Harris, Todd R
AU  - Harris TR
AD  - Department of Entomology and Cancer Research Center, University of California, 
      Davis, California 95616, USA.
FAU - Aronov, Pavel A
AU  - Aronov PA
FAU - Hammock, Bruce D
AU  - Hammock BD
LA  - eng
GR  - T32 ES007059/ES/NIEHS NIH HHS/United States
GR  - R37 ES002710-27/ES/NIEHS NIH HHS/United States
GR  - P42 ES004699/ES/NIEHS NIH HHS/United States
GR  - P42 ES004699-22/ES/NIEHS NIH HHS/United States
GR  - R37 ES02710/ES/NIEHS NIH HHS/United States
GR  - T32 ES007059-21/ES/NIEHS NIH HHS/United States
GR  - R37 ES002710/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Enzyme Inhibitors)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Enzyme Inhibitors/chemistry/pharmacology
MH  - Epoxide Hydrolases/antagonists & inhibitors/chemistry/*genetics
MH  - Evolution, Molecular
MH  - Gene Duplication
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Strongylocentrotus purpuratus/*enzymology/*genetics
PMC - PMC2858585
MID - NIHMS193790
EDAT- 2008/06/17 09:00
MHDA- 2008/09/17 09:00
PMCR- 2009/09/01
CRDT- 2008/06/17 09:00
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2008/09/17 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - 10.1089/dna.2008.0751 [pii]
AID - 10.1089/dna.2008.0751 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2008 Sep;27(9):467-77. doi: 10.1089/dna.2008.0751.