PMID- 18554572
OWN - NLM
STAT- MEDLINE
DCOM- 20090209
LR  - 20080722
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 53
IP  - 9
DP  - 2008 Sep
TI  - LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human 
      periodontal ligament cells.
PG  - 896-902
LID - 10.1016/j.archoralbio.2008.05.001 [doi]
AB  - OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptors but the 
      functional importance of oestrogen in PDL cells exposed to bacterial endotoxins 
      is not known. Here we investigate if the inflammation promoter lipopolysaccharide 
      (LPS) affects PDL cell production of interleukin-6 (IL-6), monocyte 
      chemoattractant protein-1 (MCP-1), C-reactive protein (CRP) and/or normal 
      functional PDL cell characteristics such as collagen synthesis and cell 
      proliferation and if oestrogen modulates the effects of LPS. METHODS: PDL cells 
      were obtained from periodontal ligament of premolars. PDL cells were treated with 
      Escherichia coli LPS in the absence or presence of oestrogen (17beta-oestradiol, 
      E2). Cellular concentration of IL-6, MCP-1 and CRP was determined by 
      enzyme-linked immunosorbent assay (ELISA). Collagen synthesis was determined by 
      l-[3H]proline incorporation. Cell proliferation was assessed by DNA synthesis 
      measurement using [3H]thymidine incorporation. RESULTS: Stimulation with LPS (500 
      ng/ml to 10 microg/ml) increased IL-6 production in a concentration-dependent 
      manner. Lower concentration (100 ng/ml) of LPS had no effect. LPS-induced 
      stimulation of IL-6 was not reversed by a physiologically high concentration (100 
      nM) of E2. LPS increased also MCP-1 production which was unaffected by E2. 
      Treatment with E2 alone had no effect on either IL-6 or MCP-1. Stimulation with 
      LPS had no effect on CRP. LPS did not affect collagen synthesis and cell 
      proliferation, reflecting normal physiological properties of PDL cells. 
      CONCLUSIONS: LPS stimulates PDL cell IL-6 and MCP-1 production but has no effect 
      on the normal physiological properties of PDL cells. LPS-induced IL-6 and MCP-1 
      is not reversed by oestrogen suggesting that oestrogen exerts no 
      anti-inflammatory effect via this mechanism.
FAU - Jonsson, Daniel
AU  - Jonsson D
AD  - Department of Periodontology, Faculty of Odontology, Malmo University, SE-205 06 
      Malmo, Sweden. daniel.jonsson@od.mah.se
FAU - Nebel, Daniel
AU  - Nebel D
FAU - Bratthall, Gunilla
AU  - Bratthall G
FAU - Nilsson, Bengt-Olof
AU  - Nilsson BO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080612
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Chemokine CCL2)
RN  - 0 (Estrogens)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Estrogen)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Adolescent
MH  - C-Reactive Protein/metabolism
MH  - Cells, Cultured/metabolism
MH  - Chemokine CCL2/*metabolism/pharmacology
MH  - Child
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Periodontal Ligament/drug effects/*metabolism
MH  - Receptors, Estrogen/*metabolism
EDAT- 2008/06/17 09:00
MHDA- 2009/02/10 09:00
CRDT- 2008/06/17 09:00
PHST- 2007/10/24 00:00 [received]
PHST- 2008/03/25 00:00 [revised]
PHST- 2008/05/01 00:00 [accepted]
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2009/02/10 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
AID - S0003-9969(08)00139-8 [pii]
AID - 10.1016/j.archoralbio.2008.05.001 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2008 Sep;53(9):896-902. doi: 10.1016/j.archoralbio.2008.05.001. 
      Epub 2008 Jun 12.