PMID- 18554678
OWN - NLM
STAT- MEDLINE
DCOM- 20080924
LR  - 20131121
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 231
IP  - 2
DP  - 2008 Sep 1
TI  - Styrene induces an inflammatory response in human lung epithelial cells via 
      oxidative stress and NF-kappaB activation.
PG  - 241-7
LID - 10.1016/j.taap.2008.04.010 [doi]
AB  - Styrene is a volatile organic compound (VOC) that is widely used as a solvent in 
      many industrial settings. Chronic exposure to styrene can result in irritation of 
      the mucosa of the upper respiratory tract. Contact of styrene with epithelial 
      cells stimulates the expression of a variety of inflammatory mediators, including 
      the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1). To 
      characterise the underlying mechanisms of the induction of inflammatory signals 
      by styrene, we investigated the influence of this compound on the induction of 
      oxidative stress and the activation of the nuclear factor-kappa B (NF-kappaB) 
      signalling pathway in human lung epithelial cells (A549). The results demonstrate 
      that styrene-induced MCP-1 expression, as well as the expression of the oxidative 
      stress marker glutathione S-transferase (GST), is associated with a concentration 
      dependent pattern of NF-kappaB activity. An inhibitor of NF-kappaB, IKK-NBD, and 
      the anti-inflammatory antioxidant N-acetylcysteine (NAC) were both effective in 
      suppressing styrene-induced MCP-1 secretion. In addition, NAC was capable of 
      inhibiting the upregulation of GST expression. Our findings suggest that the 
      activation of the NF-kappaB signalling pathway by styrene is mediated via a 
      redox-sensitive mechanism.
FAU - Roder-Stolinski, Carmen
AU  - Roder-Stolinski C
AD  - UFZ-Helmholtz Centre for Environmental Research, Department of Environmental 
      Immunology, Permoserstrasse 15, D-04318 Leipzig, Germany.
FAU - Fischader, Gundula
AU  - Fischader G
FAU - Oostingh, Gertie Janneke
AU  - Oostingh GJ
FAU - Feltens, Ralph
AU  - Feltens R
FAU - Kohse, Franziska
AU  - Kohse F
FAU - von Bergen, Martin
AU  - von Bergen M
FAU - Morbt, Nora
AU  - Morbt N
FAU - Eder, Klaus
AU  - Eder K
FAU - Duschl, Albert
AU  - Duschl A
FAU - Lehmann, Irina
AU  - Lehmann I
LA  - eng
PT  - Journal Article
DEP - 20080429
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (Solvents)
RN  - 44LJ2U959V (Styrene)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Glutathione Transferase/drug effects/metabolism
MH  - Humans
MH  - Lung/*drug effects/metabolism
MH  - NF-kappa B/*drug effects/metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Stress/*drug effects
MH  - Signal Transduction/drug effects
MH  - Solvents/administration & dosage/*toxicity
MH  - Styrene/administration & dosage/*toxicity
EDAT- 2008/06/17 09:00
MHDA- 2008/09/25 09:00
CRDT- 2008/06/17 09:00
PHST- 2007/11/29 00:00 [received]
PHST- 2008/03/24 00:00 [revised]
PHST- 2008/04/18 00:00 [accepted]
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2008/09/25 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
AID - S0041-008X(08)00181-6 [pii]
AID - 10.1016/j.taap.2008.04.010 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2008 Sep 1;231(2):241-7. doi: 10.1016/j.taap.2008.04.010. 
      Epub 2008 Apr 29.