PMID- 18554864
OWN - NLM
STAT- MEDLINE
DCOM- 20090106
LR  - 20220317
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 25
IP  - 4
DP  - 2008 Aug
TI  - Influence of in vitro exposure to mycotoxin zearalenone and its derivatives on 
      swine sperm quality.
PG  - 461-7
LID - 10.1016/j.reprotox.2008.04.009 [doi]
AB  - Zearalenone (ZEA) is a fusariotoxin naturally occurring in crops with known 
      estrogenic activity in swine, the most sensitive known species. The metabolism by 
      swine of ZEA, principally into alpha-zearalenol (alpha-ZOL), is considered as a 
      bio-activation because of its high affinity with estrogenic receptors. Discordant 
      data on male reproductive failures induced by ZEA in vivo are described. In this 
      study, we evaluated the effects to boar spermatozoa when they are exposed in 
      vitro to ZEA and its derivatives (alpha-ZOL, beta-ZOL). We analyzed viability, 
      apoptosis (terminal deoxynucleotidyltransferase dUTP nick end-labelling (TUNEL)), 
      sperm chromatin stability (sperm chromatin structure assay (SCSA)) and motility 
      (using computer-aided sperm analysis (CASA)). Each mycotoxin influenced a 
      specific function of spermatic cells. alpha-Zearalenol and ZEA, at picomolar 
      levels, negatively influenced chromatin structure stability and viability, 
      respectively, whereas beta-ZOL negatively influenced the sperm motility at 
      micromolar levels. This study is the first using these direct measures of sperm 
      integrity to show the potential for an adverse effect of ZEA exposure on boar 
      fertility.
FAU - Benzoni, E
AU  - Benzoni E
AD  - Department of Veterinary Science and Technologies for Food Safety, Faculty of 
      Veterinary Medicine, University of Milan, Italy.
FAU - Minervini, F
AU  - Minervini F
FAU - Giannoccaro, A
AU  - Giannoccaro A
FAU - Fornelli, F
AU  - Fornelli F
FAU - Vigo, D
AU  - Vigo D
FAU - Visconti, A
AU  - Visconti A
LA  - eng
PT  - Journal Article
DEP - 20080503
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Chromatin)
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 0 (zearalenol)
RN  - 5W827M159J (Zearalenone)
RN  - 76LO2L2V39 (Zeranol)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chromatin/drug effects
MH  - Estrogens, Non-Steroidal/*toxicity
MH  - Male
MH  - Sperm Motility/drug effects
MH  - Spermatozoa/*drug effects/physiology
MH  - Swine
MH  - Zearalenone/*toxicity
MH  - Zeranol/*analogs & derivatives/toxicity
EDAT- 2008/06/17 09:00
MHDA- 2009/01/07 09:00
CRDT- 2008/06/17 09:00
PHST- 2007/07/09 00:00 [received]
PHST- 2007/09/27 00:00 [revised]
PHST- 2008/04/24 00:00 [accepted]
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2009/01/07 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
AID - S0890-6238(08)00049-X [pii]
AID - 10.1016/j.reprotox.2008.04.009 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2008 Aug;25(4):461-7. doi: 10.1016/j.reprotox.2008.04.009. Epub 
      2008 May 3.