PMID- 18554936
OWN - NLM
STAT- MEDLINE
DCOM- 20090220
LR  - 20171116
IS  - 1522-9653 (Electronic)
IS  - 1063-4584 (Linking)
VI  - 16
IP  - 12
DP  - 2008 Dec
TI  - Differentiation potential of human muscle-derived cells towards chondrogenic 
      phenotype in alginate beads culture.
PG  - 1509-18
LID - 10.1016/j.joca.2008.04.018 [doi]
AB  - OBJECTIVE: The aim of this study was to evaluate the differentiation potential of 
      two populations of muscle-derived cells (CD56- and CD56+) towards chondrogenic 
      phenotype in alginate beads culture and to compare the effect of transforming 
      growth factor beta 1 (TGFbeta1) on the differentiation process in these 
      populations. METHODS: Muscle CD56- and CD56+ cells were cultured in alginate 
      beads, in a chondrogenic medium, containing or not TGFbeta1 (10 ng/ml). Cultures 
      were maintained for 3, 7, 14 or 21 days in a humidified culture incubator. At 
      harvest, one culture of each set was fixed for alcian blue staining and aggrecan 
      detection. The steady-state level of matrix macromolecules mRNA was assessed by 
      real-time polymerase chain reaction (PCR). Protein detection was performed by 
      western-blot analysis. The binding activity of nuclear extracts to Cbfa1 DNA 
      sequence was also evaluated by electrophoretic mobility shift assays (EMSA). 
      RESULTS: Chondrogenic differentiation of both CD56+ and CD56- muscle-derived 
      cells was improved in alginate scaffold, even without growth factor, as suggested 
      by increased chondrogenesis markers expression during the culture. Furthermore, 
      TGFbeta1 enhanced the differentiation process and allowed to maintain a high 
      expression of markers of mature chondrocytes. Of importance, the combination of 
      alginate and TGFbeta1 treatment resulted in a further down-regulation of collagen 
      type I and type X, as well as Cbfa1 both expression and binding activity. 
      CONCLUSIONS: Thus, alginate scaffold and chondrogenic medium are sufficient to 
      lead both populations CD56+ and CD56- towards chondrogenic differentiation. 
      Moreover, TGFbeta1 enhances this process and allows to maintain the chondrogenic 
      phenotype by inhibiting terminal differentiation, particularly for CD56- cells.
FAU - Andriamanalijaona, R
AU  - Andriamanalijaona R
AD  - Laboratory of Extracellular Matrix and Pathology, EA 3214, IFR 146 ICORE, 
      University of Caen Basse-Normandie, Caen Cedex, France.
FAU - Duval, E
AU  - Duval E
FAU - Raoudi, M
AU  - Raoudi M
FAU - Lecourt, S
AU  - Lecourt S
FAU - Vilquin, J T
AU  - Vilquin JT
FAU - Marolleau, J P
AU  - Marolleau JP
FAU - Pujol, J P
AU  - Pujol JP
FAU - Galera, P
AU  - Galera P
FAU - Boumediene, K
AU  - Boumediene K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080613
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Alginates)
RN  - 0 (CD56 Antigen)
SB  - IM
MH  - Alginates
MH  - CD56 Antigen/metabolism
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology
MH  - Chondrogenesis/*physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Muscle, Skeletal/*cytology/metabolism
MH  - Phenotype
EDAT- 2008/06/17 09:00
MHDA- 2009/02/21 09:00
CRDT- 2008/06/17 09:00
PHST- 2008/01/04 00:00 [received]
PHST- 2008/04/19 00:00 [accepted]
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2009/02/21 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
AID - S1063-4584(08)00135-0 [pii]
AID - 10.1016/j.joca.2008.04.018 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2008 Dec;16(12):1509-18. doi: 
      10.1016/j.joca.2008.04.018. Epub 2008 Jun 13.