PMID- 18555717
OWN - NLM
STAT- MEDLINE
DCOM- 20081209
LR  - 20200930
IS  - 1096-7184 (Electronic)
IS  - 1096-7176 (Linking)
VI  - 10
IP  - 5
DP  - 2008 Sep
TI  - Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA 
      supply.
PG  - 246-54
LID - 10.1016/j.ymben.2008.04.005 [doi]
AB  - The heterologous production of fungal polyketides was investigated using 
      6-methylsalicylic acid synthase (6-MSAS) as a model polyketide synthase and 
      Saccharomyces cerevisiae as a host. In order to improve the production of 6-MSA 
      by enhancing the supply of precursors, the promoter of the gene (ACC1) encoding 
      acetyl-CoA carboxylase, which catalyzes the conversion of acetyl-CoA to 
      malonyl-CoA, was replaced with a strong, constitutive promoter (TEF1p) in a 
      strain harboring two plasmids carrying the genes encoding 6-MSAS from Penicillium 
      patulum and PPTase from Aspergillus nidulans, respectively. The strain was 
      characterized in batch cultivations with a glucose minimal media (20 g/L), and a 
      60% increase in 6-MSA titer was observed compared to a strain having the native 
      promoter in front of ACC1. The production of 6-MSA was scaled up by the 
      cultivation in minimal media containing 50 g/L of glucose, and hereby a final 
      titer of 554+/-26 mg/L of 6-MSA was obtained.
FAU - Wattanachaisaereekul, Songsak
AU  - Wattanachaisaereekul S
AD  - Center for Microbial Biotechnology, Technical University of Denmark, Department 
      of Systems Biology, Denmark.
FAU - Lantz, Anna Eliasson
AU  - Lantz AE
FAU - Nielsen, Michael Lynge
AU  - Nielsen ML
FAU - Nielsen, Jens
AU  - Nielsen J
LA  - eng
PT  - Journal Article
DEP - 20080510
PL  - Belgium
TA  - Metab Eng
JT  - Metabolic engineering
JID - 9815657
RN  - 0 (Macrolides)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Peptide Elongation Factor 1)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Salicylates)
RN  - 0 (TEF1 protein, S cerevisiae)
RN  - 524-14-1 (Malonyl Coenzyme A)
RN  - 72-89-9 (Acetyl Coenzyme A)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.- (aminoglycoside N1-acetyltransferase)
RN  - EC 6.- (6-methylsalicylic acid synthetase)
RN  - EC 6.- (Ligases)
RN  - L5352FE23Y (6-methylsalicylic acid)
SB  - IM
MH  - Acetyl Coenzyme A/genetics/metabolism
MH  - Acetyltransferases/*biosynthesis/genetics
MH  - Acyltransferases/*biosynthesis/genetics
MH  - Aspergillus nidulans/enzymology/genetics
MH  - Ligases/*biosynthesis/genetics
MH  - Macrolides/*metabolism
MH  - Malonyl Coenzyme A/genetics/metabolism
MH  - Multienzyme Complexes/*biosynthesis/genetics
MH  - Oxidoreductases/*biosynthesis/genetics
MH  - Penicillium/enzymology/genetics
MH  - Peptide Elongation Factor 1/genetics/*metabolism
MH  - Saccharomyces cerevisiae/*enzymology/genetics/growth & development
MH  - Saccharomyces cerevisiae Proteins/genetics/*metabolism
MH  - Salicylates/*metabolism
EDAT- 2008/06/17 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/17 09:00
PHST- 2007/10/01 00:00 [received]
PHST- 2008/02/04 00:00 [revised]
PHST- 2008/04/28 00:00 [accepted]
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
AID - S1096-7176(08)00019-0 [pii]
AID - 10.1016/j.ymben.2008.04.005 [doi]
PST - ppublish
SO  - Metab Eng. 2008 Sep;10(5):246-54. doi: 10.1016/j.ymben.2008.04.005. Epub 2008 May 
      10.