PMID- 18557960
OWN - NLM
STAT- MEDLINE
DCOM- 20100222
LR  - 20090116
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 105
IP  - 5
DP  - 2008 Nov
TI  - Ecophysiology of polyphosphate-accumulating organisms and glycogen-accumulating 
      organisms in a continuously aerated enhanced biological phosphorus removal 
      process.
PG  - 1412-20
LID - 10.1111/j.1365-2672.2008.03857.x [doi]
AB  - AIMS: To investigate the ecophysiology of populations of 
      polyphosphate-accumulating organisms (PAO) and glycogen-accumulating organisms 
      (GAO) in communities of a novel acetate fed process removing phosphate from 
      wastewater. Attempts were made to see if acetate could be replaced by an 
      alternative carbon source which did not support the growth of the GAO. METHODS 
      AND RESULTS: A continuously aerated sequencing batch reactor was operated with 
      different acetate feed levels. Fluorescence in situ hybridization (FISH) showed 
      that Defluviicoccus GAO numbers increased at lower acetate feed levels. With 
      FISH/microautoradiography (MAR) both detected morphotypes of Defluviicoccus 
      assimilated a wider range of substrates aerobically than Accumulibacter PAO. 
      Their uptake profile differed from that reported for the same phylotype in full 
      scale anaerobic : aerobic EBPR plants. CONCLUSIONS: This suggests that replacing 
      acetate with another substrate is unlikely to provide Accumulibacter with a 
      selective advantage in this process. Why Defluviicoccus appeared to out-compete 
      Accumulibacter at lower acetate concentrations was not clear. Data suggest 
      physiological and morphological diversity may exist within a single 
      Defluviicoccus phylotype. SIGNIFICANCE AND IMPACT OF THE STUDY: This study 
      implies that the current FISH probes for Defluviicoccus GAO may not reveal the 
      full extent of their biodiversity, and that more information is required before 
      strategies for their control can be devised.
FAU - Schroeder, S
AU  - Schroeder S
AD  - Biotechnology Research Centre, La Trobe University, Bendigo, Vic., Australia.
FAU - Ahn, J
AU  - Ahn J
FAU - Seviour, R J
AU  - Seviour RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080614
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Acetates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Acetates/*metabolism
MH  - Aerobiosis
MH  - Alphaproteobacteria/*metabolism
MH  - Betaproteobacteria/*metabolism
MH  - Bioreactors/*microbiology
MH  - *Ecology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Glycogen/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Waste Disposal, Fluid
MH  - *Water Microbiology
EDAT- 2008/06/19 09:00
MHDA- 2010/02/23 06:00
CRDT- 2008/06/19 09:00
PHST- 2008/06/19 09:00 [pubmed]
PHST- 2010/02/23 06:00 [medline]
PHST- 2008/06/19 09:00 [entrez]
AID - JAM3857 [pii]
AID - 10.1111/j.1365-2672.2008.03857.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2008 Nov;105(5):1412-20. doi: 10.1111/j.1365-2672.2008.03857.x. 
      Epub 2008 Jun 14.