PMID- 18558287
OWN - NLM
STAT- MEDLINE
DCOM- 20080807
LR  - 20080618
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 184
IP  - 1
DP  - 2008 Jul
TI  - Characterization of homozygous deletions in laryngeal squamous cell carcinoma 
      cell lines.
PG  - 38-43
LID - 10.1016/j.cancergencyto.2008.03.004 [doi]
AB  - The majority of classical tumor suppressor genes, such as CDKN2A or RB1, were 
      identified by delineation of biallelic losses called homozygous deletions. To 
      systematically identify homozygous deletions in laryngeal squamous cell carcinoma 
      and to unravel novel putative tumor suppressor genes we screened three laryngeal 
      squamous cell carcinoma cell lines (LSCC) using array comparative genomic 
      hybridization (array-CGH). Out of 31 candidate regions for homozygous deletions 
      identified by array-CGH, 5 were verified further by PCR. Among others, these 
      homozygous deletions affected the tumor suppressor gene CDKN2A and the 
      apoptosis-inducing STK17A gene. To assess the frequency of the identified 
      deletions we investigated the affected sites in 9 additional LSCC cell lines. In 
      5 of the 9 cell lines the CDKN2A gene was homozygously lost. Thus, CDKN2A was 
      homozygously deleted in 7 of the 12 cell lines. No other recurrent homozygous 
      deletions were found. Homozygous deletions was a frequent mechanism of CDKN2A 
      inactivation. Moreover, we identified several other genes, including the putative 
      tumor suppressor gene STK17A, which may be inactivated by homozygous deletions 
      and thus are potentially implicated in laryngeal squamous cell carcinoma 
      development.
FAU - Giefing, Maciej
AU  - Giefing M
AD  - Institute of Human Genetics, Polish Academy of Sciences, ul. Strzeszynska 32, 
      60-479 Poznan, Poland. gifciu@wp.pl
FAU - Martin-Subero, Jose Ignacio
AU  - Martin-Subero JI
FAU - Kiwerska, Katarzyna
AU  - Kiwerska K
FAU - Jarmuz, Malgorzata
AU  - Jarmuz M
FAU - Grenman, Reidar
AU  - Grenman R
FAU - Siebert, Reiner
AU  - Siebert R
FAU - Szyfter, Krzysztof
AU  - Szyfter K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - DNA Primers
MH  - *Homozygote
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction
EDAT- 2008/06/19 09:00
MHDA- 2008/08/08 09:00
CRDT- 2008/06/19 09:00
PHST- 2007/08/23 00:00 [received]
PHST- 2008/02/13 00:00 [revised]
PHST- 2008/03/03 00:00 [accepted]
PHST- 2008/06/19 09:00 [pubmed]
PHST- 2008/08/08 09:00 [medline]
PHST- 2008/06/19 09:00 [entrez]
AID - S0165-4608(08)00192-1 [pii]
AID - 10.1016/j.cancergencyto.2008.03.004 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Jul;184(1):38-43. doi: 
      10.1016/j.cancergencyto.2008.03.004.