PMID- 18561938
OWN - NLM
STAT- MEDLINE
DCOM- 20080924
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1201
IP  - 1
DP  - 2008 Aug 1
TI  - Single cell determination of nitric oxide release using capillary electrophoresis 
      with laser-induced fluorescence detection.
PG  - 120-7
LID - 10.1016/j.chroma.2008.06.001 [doi]
AB  - Measurements of nitric oxide (NO) release at single cell level are fundamental to 
      understand the diverse physiological functions of this remarkable molecule. To 
      achieve this purpose, capillary electrophoresis with laser-induced fluorescence 
      detection (CE-LIF) was originally described for the sensitive determination of NO 
      release in individual neuron and mammalian cell after 
      8-(3,4-diaminophenyl)-2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene 
      (DAMBO-P(H)) was chosen as the fluorescent probe. Various parameters affecting NO 
      trapping in vivo and CE separation were systematically studied. Under the optimal 
      conditions, complete and fast separation of the resulted targeted 
      high-fluorescent triazole (DAMBO-P(H)-T) was achieved in about 3 min (2.89 min), 
      and the relative standard deviations (RSDs) values of migration time and peak 
      area were less than 5% and 9% for intra-day and inter-day assays, respectively. 
      The detection limit was 42 amol (at a signal-to-noise ratio of 3). The 
      feasibility of application of the developed method was validated by successfully 
      applied to the measurements of NO release from four single cell study models. 
      This original application of this method in diverse samples represents a powerful 
      tool to study the kinetics of NO release by neuronal cells during 
      neurotransmission, as well as for the understanding of the pathobiological and 
      therapeutic basis of this molecule for cardiovascular diseases and under 
      oxidative stress.
FAU - Yang, Qiao
AU  - Yang Q
AD  - Department of Biochemistry and Molecular Biology, College of Basic Medical 
      Sciences, Second Military Medical University, Shanghai 200433, China. 
      qyangsmmu@gmail.com
FAU - Zhang, Xiaoling
AU  - Zhang X
FAU - Bao, Xiaohui
AU  - Bao X
FAU - Lu, Hongjuan
AU  - Lu H
FAU - Zhang, Wenjun
AU  - Zhang W
FAU - Wu, Wenhui
AU  - Wu W
FAU - Miao, Huinan
AU  - Miao H
FAU - Jiao, Binghua
AU  - Jiao B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080606
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dogs
MH  - Electrophoresis, Capillary/*methods
MH  - Endothelium/cytology/metabolism
MH  - Ganglia/cytology/metabolism
MH  - Humans
MH  - Microchemistry/*methods
MH  - Neurons/cytology/metabolism
MH  - Nitric Oxide/*analysis
MH  - Rats
MH  - Snails
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2008/06/20 09:00
MHDA- 2008/09/25 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/03/17 00:00 [received]
PHST- 2008/05/29 00:00 [revised]
PHST- 2008/06/02 00:00 [accepted]
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/09/25 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - S0021-9673(08)00986-2 [pii]
AID - 10.1016/j.chroma.2008.06.001 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Aug 1;1201(1):120-7. doi: 10.1016/j.chroma.2008.06.001. Epub 
      2008 Jun 6.