PMID- 18562583
OWN - NLM
STAT- MEDLINE
DCOM- 20090924
LR  - 20220408
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 46
IP  - 9
DP  - 2008 Sep
TI  - Evidence of multiple Treponema phylotypes involved in bovine digital dermatitis 
      as shown by 16S rRNA gene analysis and fluorescence in situ hybridization.
PG  - 3012-20
LID - 10.1128/JCM.00670-08 [doi]
AB  - The etiopathogenesis of the skin disease digital dermatitis (DD), an important 
      cause of lameness in cattle, remains uncertain. Microscopically, the disease 
      appears to be polymicrobial, with spirochetes as the predominant bacteria. The 
      objective of this study was to identify the main part of the bacteria involved in 
      DD lesions of cattle by using culture-independent molecular methods. Ten 
      different phylotypes of Treponema were identified either by 16S rRNA gene 
      sequencing of bacteria from DD lesions or by fluorescence in situ hybridization 
      (FISH) analysis using phylotype-specific 16S rRNA-directed oligonucleotide 
      probes. Two phylotypes, phylotype 1 (PT1) and PT2, were not closely related to 
      any characterized treponemal species. PT7 was 99.3% identical to Treponema 
      denticola, while PT9 resembled T. vincentii by 96%. The remaining phylotypes, 
      PT3, PT4, PT5, PT6, and PT8, and Treponema brennaborense had previously been 
      isolated from DD lesions. Forty DD biopsy specimens were examined for Treponema 
      by FISH. With one exception, all of the biopsy specimens revealed epidermotropic, 
      intermingled infection with three or more different phylotypes (mean, 4.7). The 
      most prevalent species were PT1 (95%), PT6 (93%), and PT3 (85%). While 
      colonization by PT3 was confined to the surface of the epidermis, both PT1 and 
      PT6 invaded deep into the stratum spinosum and were seen in ulcerated dermal 
      papillae. In two cases, all 10 phylotypes were demonstrated. Furthermore, FISH 
      with a Treponema group-specific probe showed that Treponema accounted for more 
      than 90% of the total bacterial population in the biopsy specimens. These data 
      strongly suggest that a group of apparently symbiotic Treponema species are 
      involved as primary bacterial pathogens in DD.
FAU - Klitgaard, Kirstine
AU  - Klitgaard K
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1790 Copenhagen V, Denmark.
FAU - Boye, Mette
AU  - Boye M
FAU - Capion, Nynne
AU  - Capion N
FAU - Jensen, Tim K
AU  - Jensen TK
LA  - eng
SI  - GENBANK/AM942445
SI  - GENBANK/AM942446
SI  - GENBANK/AM942447
SI  - GENBANK/AM942448
SI  - GENBANK/AM942449
SI  - GENBANK/AM942450
SI  - GENBANK/AM942451
SI  - GENBANK/AM980447
SI  - GENBANK/AM980448
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080618
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*microbiology
MH  - Dermatitis/*veterinary
MH  - Foot Diseases/microbiology/*veterinary
MH  - Hindlimb/microbiology
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes/genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Treponema/*isolation & purification
MH  - Treponemal Infections/microbiology/*veterinary
PMC - PMC2546760
EDAT- 2008/06/20 09:00
MHDA- 2009/09/25 06:00
PMCR- 2008/12/18
CRDT- 2008/06/20 09:00
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2009/09/25 06:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
PHST- 2008/12/18 00:00 [pmc-release]
AID - JCM.00670-08 [pii]
AID - 0670-08 [pii]
AID - 10.1128/JCM.00670-08 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2008 Sep;46(9):3012-20. doi: 10.1128/JCM.00670-08. Epub 2008 
      Jun 18.