PMID- 18563285
OWN - NLM
STAT- MEDLINE
DCOM- 20081218
LR  - 20131121
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 153
IP  - 8
DP  - 2008
TI  - Porcine reproductive and respiratory syndrome virus modulates apoptosis during 
      replication in alveolar macrophages.
PG  - 1453-65
LID - 10.1007/s00705-008-0135-5 [doi]
AB  - Different viruses have evolved strategies that inhibit apoptosis of the host cell 
      early in infection and/or induce apoptosis in the host cell late in infection. In 
      this study, it was investigated if and when porcine reproductive and respiratory 
      syndrome virus (PRRSV) modulates apoptosis in PRRSV-infected macrophages. The 
      PRRSV replication cycle in macrophages was completed within 12 h post-inoculation 
      (hpi). PRRSV-infected macrophages, treated with staurosporine at 4, 5, 6 and 8 
      hpi, were significantly protected against staurosporine-induced apoptosis, but 
      PRRSV-infected macrophages, treated with staurosporine at 12 hpi, were not. In 
      contrast, starting from 12 hpi, all PRRSV-infected macrophages died by 
      caspase-dependent apoptosis, which culminated in secondary necrosis. Treatment of 
      PRRSV-infected macrophages with Z-Val-DL-Asp-fluoromethylketone indicated that 
      apoptosis late in infection was not essential for efficient virus release. Anti- 
      and pro-apoptotic activities were also observed in PRRSV-infected Marc-145 cells. 
      In conclusion, this study shows that PRRSV stimulates anti-apoptotic pathways in 
      macrophages early in infection and that PRRSV-infected macrophages die by 
      apoptosis late in infection.
FAU - Costers, Sarah
AU  - Costers S
AD  - Department of Virology, Parasitology and Immunology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, Merelbeke, Belgium. 
      sarah.costers@ugent.be
FAU - Lefebvre, David J
AU  - Lefebvre DJ
FAU - Delputte, Peter L
AU  - Delputte PL
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080619
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology/*physiology
MH  - Macrophages, Alveolar/drug effects/immunology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Staurosporine/pharmacology
MH  - Swine Diseases/pathology/virology
MH  - Virus Replication/*physiology
EDAT- 2008/06/20 09:00
MHDA- 2008/12/19 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/04/03 00:00 [received]
PHST- 2008/05/10 00:00 [accepted]
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/12/19 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - 10.1007/s00705-008-0135-5 [doi]
PST - ppublish
SO  - Arch Virol. 2008;153(8):1453-65. doi: 10.1007/s00705-008-0135-5. Epub 2008 Jun 
      19.