PMID- 18563337
OWN - NLM
STAT- MEDLINE
DCOM- 20081126
LR  - 20211203
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 28
IP  - 3
DP  - 2008 Jun
TI  - The expression of mammalian target of rapamycin in Ishikawa and HEC-1A cells.
PG  - 340-2
LID - 10.1007/s11596-008-0325-0 [doi]
AB  - The activation of mammalian target of rapamycin (mTOR) signaling pathway in 
      endometrial carcinoma cells Ishikawa and HEC-1A was investigated. The expression 
      of mTOR was detected by confocal fluorescence microscopy in Ishikawa and HEC-1A 
      cells. The mRNA levels of PTEN and mTOR, the downstream substrate S6K1 and 4E-BP1 
      protein were assayed by RT-PCR and Western blot, respectively. The expression of 
      PTEN in Ishikawa cells was deficient, but intact in HEC-1A cells respectively 
      (P<0.01). There was mTOR expression in both Ishikawa and HEC-1A cells and the 
      phosphorylated substrate levels in Ishikawa cells were higher than those in 
      HEC-1A cells (P<0.05). mTOR signaling pathway is activated in two endometrial 
      carcinoma cell strains and the status of activation is related with PTEN 
      expression of the cells. The activation level of mTOR is higher in PTEN-deficient 
      endometrial carcinoma cells than that in PTEN-intact endometrial carcinoma cells.
FAU - Li, Xiaomao
AU  - Li X
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430022, China.
FAU - Xiao, Lan
AU  - Xiao L
FAU - Yang, Yuebo
AU  - Yang Y
FAU - Shen, Huimin
AU  - Shen H
FAU - Zeng, Haitao
AU  - Zeng H
FAU - Wang, Zehua
AU  - Wang Z
LA  - eng
PT  - Journal Article
DEP - 20080619
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Phosphoproteins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/biosynthesis
MH  - Apoptosis
MH  - Carcinoma/metabolism
MH  - Cell Cycle Proteins
MH  - Cell Line, Tumor
MH  - Endometrial Neoplasms/*metabolism/pathology
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Microscopy, Confocal
MH  - PTEN Phosphohydrolase/*biosynthesis/metabolism
MH  - Phosphoproteins/biosynthesis
MH  - Phosphorylation
MH  - Protein Kinases/*biosynthesis/metabolism/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribosomal Protein S6 Kinases/biosynthesis
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases
EDAT- 2008/06/20 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/03/10 00:00 [received]
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - 10.1007/s11596-008-0325-0 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2008 Jun;28(3):340-2. doi: 
      10.1007/s11596-008-0325-0. Epub 2008 Jun 19.