PMID- 18563361
OWN - NLM
STAT- MEDLINE
DCOM- 20080919
LR  - 20131121
IS  - 0253-6269 (Print)
IS  - 0253-6269 (Linking)
VI  - 31
IP  - 6
DP  - 2008 Jun
TI  - Effect of proton beam on blood vessel formation in early developing zebrafish 
      (Danio rerio) embryos.
PG  - 779-85
LID - 10.1007/s12272-001-1226-1 [doi]
AB  - Proton beam therapy can kill tumor cells while saving normal cells because of its 
      specific energy delivery properties and so is used to various tumor patients. 
      However, the effect of proton beam on angiogenesis in the development of blood 
      vessels has not been determined. Here we used the zebrafish model to determine in 
      vivo whether proton beam inhibits angiogenesis. Flk-1-GFP transgenic embryos 
      irradiated with protons (35 MeV, spread out Bragg peak, SOBP) demonstrated a 
      marked inhibition of embryonic growth and an altered fluorescent blood vessel 
      development in the trunk region. When cells were stained with acridine orange to 
      evaluate DNA damage, the number of green fluorescent cell death spots was 
      increased in trunk regions of irradiated embryos compared to non-irradiated 
      control embryos. Proton beam also significantly increased the cell death rate in 
      human umbilical vein endothelial cells (HUVEC), but pretreatment with N-acetyl 
      cystein (NAC), an antioxidant, reduced the proton-induced cell death rate 
      (p<0.01). Moreover, pretreatment with NAC abrogated the inhibition of trunk 
      vessel development and prevented the trunk malformation caused by proton 
      irradiation. In conclusion, proton irradiation significantly inhibited in vivo 
      vascular development possibly due to increased vascular cell death via reactive 
      oxygen species formation.
FAU - Jang, Gun Hyuk
AU  - Jang GH
AD  - School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook 
      National University, Daegu, 702-701, Korea.
FAU - Ha, Ji-Hong
AU  - Ha JH
FAU - Huh, Tae-Lin
AU  - Huh TL
FAU - Lee, You Mie
AU  - Lee YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080619
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Antioxidants)
RN  - 0 (Protons)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Antioxidants/pharmacology
MH  - Blood Vessels/drug effects/embryology/metabolism/*radiation effects
MH  - Cell Death/radiation effects
MH  - Cells, Cultured
MH  - DNA Damage
MH  - Dose-Response Relationship, Radiation
MH  - Endothelial Cells/metabolism/pathology/radiation effects
MH  - Green Fluorescent Proteins/metabolism
MH  - Humans
MH  - Neovascularization, Physiologic/drug effects/*radiation effects
MH  - *Protons
MH  - Reactive Oxygen Species/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Vascular Endothelial Growth Factor Receptor-2/genetics/metabolism
MH  - Zebrafish/*embryology/genetics/metabolism
EDAT- 2008/06/20 09:00
MHDA- 2008/09/20 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/04/01 00:00 [received]
PHST- 2008/05/08 00:00 [accepted]
PHST- 2008/04/13 00:00 [revised]
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/09/20 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - 10.1007/s12272-001-1226-1 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2008 Jun;31(6):779-85. doi: 10.1007/s12272-001-1226-1. Epub 2008 
      Jun 19.