PMID- 18563365 OWN - NLM STAT- MEDLINE DCOM- 20080919 LR - 20151119 IS - 0253-6269 (Print) IS - 0253-6269 (Linking) VI - 31 IP - 6 DP - 2008 Jun TI - Simultaneous determination of amphetamine-type stimulants and cannabinoids in fingernails by gas chromatography-mass spectrometry. PG - 805-13 LID - 10.1007/s12272-001-1230-5 [doi] AB - A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the simultaneous detection and quantification of four amphetamine-type stimulants (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)) and two cannabinoids (Delta9-tetrahydrocannabinol (Delta9-THC) and 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid (THCCOOH)) in fingernails. Fingernail clippings (30 mg) were washed with distilled water and methanol, and then incubated in 1.0 M sodium hydroxide at 95 degrees C for 30 min. The compounds of interest were isolated by liquid-liquid extraction followed by derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) at 70 degrees C for 15 min. The derivatized compounds were analyzed by GC-MS in the selective ion monitoring (SIM) mode. The linear ranges were 0.1-15.0 ng/mg for AP, 0.2-15.0 ng/mg for MDA, Delta9-THC and THCCOOH, and 0.2-30.0 ng/mg for MA and MDMA, with good correlation coefficients (r2 > 0.9991). The intra-day, inter-day, and inter-person precisions were within 10.6%, 6.3%, and 5.3%, respectively. The intra-day, inter-day and inter-person accuracies were between -6.1 and 5.0%, -6.2 and 5.7%, and -6.4 and 5.6%, respectively. The limits of detection (LODs) and quantification (LOQs) for each compound were lower than 0.056 and 0.2 ng/mg, respectively. The recoveries were in the range of 74.0-94.8%. Positive GC-MS results were obtained from specimens of nine suspected MA or cannabis abusers. The concentration ranges of MA, AP, and THCCOOH were 0.10-1.41, 0.12-2.64, and 0.20 ng/mg, respectively. Based on these results, the method proved to be effective for the simultaneous qualification and quantification of amphetamine-type stimulants and cannabinoids in fingernails. FAU - Kim, Jin Young AU - Kim JY AD - Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, 706, Banporo, Seocho-gu, Seoul, 137-730, Korea. paxus@spo.go.kr FAU - Cheong, Jae Chul AU - Cheong JC FAU - Kim, Min Kyoung AU - Kim MK FAU - Lee, Jae Il AU - Lee JI FAU - In, Moon Kyo AU - In MK LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080619 PL - Korea (South) TA - Arch Pharm Res JT - Archives of pharmacal research JID - 8000036 RN - 0 (Amphetamines) RN - 44RAL3456C (Methamphetamine) RN - 4764-17-4 (3,4-Methylenedioxyamphetamine) RN - 4TPC9E4A32 (11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid) RN - 7J8897W37S (Dronabinol) RN - CK833KGX7E (Amphetamine) RN - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine) SB - IM MH - 3,4-Methylenedioxyamphetamine/analysis MH - Amphetamine/analysis MH - Amphetamine-Related Disorders/*diagnosis/metabolism MH - Amphetamines/*analysis MH - Dronabinol/analogs & derivatives/*analysis MH - *Gas Chromatography-Mass Spectrometry MH - Humans MH - Marijuana Abuse/*diagnosis/metabolism MH - Methamphetamine/analysis MH - N-Methyl-3,4-methylenedioxyamphetamine/analysis MH - Nails/*chemistry MH - Observer Variation MH - Reproducibility of Results MH - Substance Abuse Detection/*methods EDAT- 2008/06/20 09:00 MHDA- 2008/09/20 09:00 CRDT- 2008/06/20 09:00 PHST- 2008/02/12 00:00 [received] PHST- 2008/04/15 00:00 [accepted] PHST- 2008/03/10 00:00 [revised] PHST- 2008/06/20 09:00 [pubmed] PHST- 2008/09/20 09:00 [medline] PHST- 2008/06/20 09:00 [entrez] AID - 10.1007/s12272-001-1230-5 [doi] PST - ppublish SO - Arch Pharm Res. 2008 Jun;31(6):805-13. doi: 10.1007/s12272-001-1230-5. Epub 2008 Jun 19.