PMID- 18563422
OWN - NLM
STAT- MEDLINE
DCOM- 20081202
LR  - 20131121
IS  - 1432-069X (Electronic)
IS  - 0340-3696 (Linking)
VI  - 300
IP  - 8
DP  - 2008 Sep
TI  - Cytokine mRNA levels in human fat tissue after injection lipolysis with 
      phosphatidylcholine and deoxycholate.
PG  - 455-9
LID - 10.1007/s00403-008-0869-7 [doi]
AB  - Injections with Lipostabil, a phosphatidylcholine and deoxycholate containing 
      substance, have become a popular technique to treat localized fat accumulation 
      and lipomas. It is believed that the injected substance leads to fat cell 
      destruction with subsequent acute panniculitis followed by a repair process of 
      the treated fat tissue. We sought to investigate the mRNA expression of cytokines 
      within the acute stage of panniculitis following Lipostabil injections. Tumor 
      necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-2 
      (IL-2), IL-4, IL-5, IL-6, IL-8, and IL-10 mRNA expression were determined using 
      quantitative real-time reverse transcriptase polymerase chain reaction in treated 
      and adjacent non-treated fat tissue of lipomas 48 h after injection in seven 
      patients. Following injection lipolysis with Lipostabil, TNF-alpha, IL-6, IL-8, 
      and IL-10 mRNA levels were significantly elevated in treated fat tissue compared 
      to non-treated fat. No significant differences were found for IL-4. Both in 
      treated and non-treated fat tissue, mRNA of IFN-gamma, IL-2, and IL-5 was not 
      expressed. Injection lipolysis induces acute panniculitis within the treated fat 
      tissue, associated with changes of the cytokine profile. However, their 
      pathogenetic relevance with respect to clinical dissolving of fat needs further 
      investigation.
FAU - Bechara, Falk G
AU  - Bechara FG
AD  - Department of Dermatology and Allergology, Ruhr-University Bochum, Bochum, 
      Germany. f.bechara@derma.de
FAU - Skrygan, Marina
AU  - Skrygan M
FAU - Kreuter, Alexander
AU  - Kreuter A
FAU - Altmeyer, Peter
AU  - Altmeyer P
FAU - Gambichler, Thilo
AU  - Gambichler T
LA  - eng
PT  - Journal Article
DEP - 20080618
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Cytokines)
RN  - 0 (Fat Emulsions, Intravenous)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (RNA, Messenger)
RN  - 005990WHZZ (Deoxycholic Acid)
RN  - 11096-62-1 (lipostabil)
SB  - IM
MH  - Adipose Tissue/*metabolism
MH  - Adult
MH  - Cytokines/*genetics
MH  - Deoxycholic Acid/pharmacology
MH  - Fat Emulsions, Intravenous/*pharmacology
MH  - Female
MH  - Humans
MH  - Injections
MH  - Lipolysis/*drug effects
MH  - Male
MH  - Phosphatidylcholines/*pharmacology
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/06/20 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/03/23 00:00 [received]
PHST- 2008/05/28 00:00 [accepted]
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - 10.1007/s00403-008-0869-7 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 2008 Sep;300(8):455-9. doi: 10.1007/s00403-008-0869-7. Epub 
      2008 Jun 18.