PMID- 18563705
OWN - NLM
STAT- MEDLINE
DCOM- 20090807
LR  - 20131121
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 76
IP  - 3
DP  - 2009 Mar
TI  - Regulatory changes of apoptotic factors in the bovine corpus luteum after induced 
      luteolysis.
PG  - 220-30
LID - 10.1002/mrd.20946 [doi]
AB  - The corpus luteum (CL) offers the opportunity to study not only proliferative, 
      but also regressive processes. During luteolysis of the CL a sudden death of 
      luteal and endothelial cells seems to be involved (apoptosis). The aim of this 
      study was to examen the mRNA expression of factors known to be involved in 
      apoptotic processes: monocyte chemoattractant protein-1 (MCP-1), factors of the 
      extrinsic and intrinsic apoptotic pathways, caspase3, -6, -7 and interferone 
      gamma (IFNgamma). Luteolysis was induced by injection of 500 microg Cloprostenol 
      during mid-luteal phase. The CLs were collected at 0.5, 2, 4, 12, 24, 48, and 64 
      hr after PGF2alpha-injection. Control CLs (Days 8-12) were collected at the 
      slaugtherhouse. Real-time RT-PCR determined the mRNA expressions. Western blot 
      analysis of poly(ADP-ribose) polymerase (PARP-1) and IFNgamma as well as protein 
      measurement of tumor necrosis factor alpha (TNFalpha) by EIA were performed. The 
      mRNA levels of MCP-1, IFNgamma and most factors of the extrinsic pathway were 
      significantly increased between 0.5 and 2 hr. The factors of the intrinsic 
      pathway were mostly later up-regulated at 24-48 hr after PGF2alpha. Caspase6 and 
      3 revealed a significant increase from 2 and 12 hr, respectively, whereas 
      caspase7 was significantly up-regulated after 24 hr. The protein level of 
      TNFalpha increased significantly to a maximum level at 12 hr. The Western blot 
      revealed an increasing level of an 89 kDa fragment of PARP-1 from 12 to 24 hr, 
      which is specific for apoptosis. We assume that the extrinsic pathway is more 
      important for the onset of luteolysis, because of its earlier and higher increase 
      during induced luteolysis.
FAU - Kliem, H
AU  - Kliem H
AD  - Physiology Weihenstephan, Technical University Munich, Freising, Germany.
FAU - Berisha, B
AU  - Berisha B
FAU - Meyer, H H D
AU  - Meyer HH
FAU - Schams, D
AU  - Schams D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (CD163 antigen)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Luteolytic Agents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 4208238832 (Cloprostenol)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - Apoptosis/physiology
MH  - Apoptosis Regulatory Proteins/*genetics/*metabolism
MH  - Caspases/genetics/metabolism
MH  - Cattle
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cloprostenol/pharmacology
MH  - Corpus Luteum/chemistry
MH  - Female
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Interferon-gamma/genetics/metabolism
MH  - Luteolysis/drug effects/*physiology
MH  - Luteolytic Agents/pharmacology
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Progesterone/blood
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - Receptors, CCR2/genetics/metabolism
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - Tumor Suppressor Protein p53/genetics/metabolism
EDAT- 2008/06/20 09:00
MHDA- 2009/08/08 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2009/08/08 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - 10.1002/mrd.20946 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2009 Mar;76(3):220-30. doi: 10.1002/mrd.20946.