PMID- 18564036
OWN - NLM
STAT- MEDLINE
DCOM- 20081208
LR  - 20181201
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 17
IP  - 5
DP  - 2008 Oct
TI  - Neuronal differentiation potential of human adipose-derived mesenchymal stem 
      cells.
PG  - 909-16
LID - 10.1089/scd.2007.0197 [doi]
AB  - Adult mesenchymal stem cells derived from adipose tissue (A-MSC) have the 
      capacity to differentiate in vitro into mesenchymal as well as endodermal and 
      ectodermal cell lineages. We investigated the neuronal differentiation potential 
      of human A-MSC with a protocol which included sphere formation and sequential 
      culture in brain-derived neurotrophic factor (BDNF) and retinoic acid (RA). After 
      30 days, about 57% A-MSC showed morphological, immunocytochemical and 
      electrophysiological evidence of initial neuronal differentiation. In fact, A-MSC 
      displayed elongated shape with protrusion of two or three cellular processes, 
      selectively expressed nestin and neuronal molecules (including GABA receptor and 
      tyroxine hydroxilase) in the absence of glial phenotypic markers. Differentiated 
      cells showed negative membrane potential (-60 mV), delayed rectifier potassium 
      currents and TTX-sensitive sodium currents. Such changes were stable for at least 
      7 days after removal of differentiation medium. In view of these results and the 
      easy availability of adipose tissue, A-MSC may be a ready source of adult MSC 
      with neuronal differentiation potential, an useful tool to treat 
      neurodegenerative diseases.
FAU - Anghileri, Elena
AU  - Anghileri E
AD  - Department of Neurological Sciences and Vision, University of Verona, Verona, 
      Italy.
FAU - Marconi, Silvia
AU  - Marconi S
FAU - Pignatelli, Angela
AU  - Pignatelli A
FAU - Cifelli, Pierangelo
AU  - Cifelli P
FAU - Galie, Mirco
AU  - Galie M
FAU - Sbarbati, Andrea
AU  - Sbarbati A
FAU - Krampera, Mauro
AU  - Krampera M
FAU - Belluzzi, Ottorino
AU  - Belluzzi O
FAU - Bonetti, Bruno
AU  - Bonetti B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - *Cell Differentiation
MH  - Cell Shape
MH  - Electrophysiology
MH  - Female
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/ultrastructure
MH  - Neurons/*cytology/ultrastructure
EDAT- 2008/06/20 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - 10.1089/scd.2007.0197 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2008 Oct;17(5):909-16. doi: 10.1089/scd.2007.0197.