PMID- 18564223
OWN - NLM
STAT- MEDLINE
DCOM- 20081028
LR  - 20161018
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 22
IP  - 4
DP  - 2008 Jul-Aug
TI  - Search for Borrelia burgdorferi in kidneys of dogs with suspected "Lyme 
      nephritis".
PG  - 860-5
LID - 10.1111/j.1939-1676.2008.0131.x [doi]
AB  - BACKGROUND: "Lyme nephritis" is a poorly characterized condition associated with 
      proteinuria and often fatal renal failure in dogs with serological evidence of 
      infection with Borrelia burgdorferi. OBJECTIVE: The aim of this study was to 
      determine if intact B. burgdorferi organisms were present in the kidneys of 
      serologically Lyme-positive dogs with histopathologic features of Lyme nephritis. 
      ANIMALS: Twenty-six affected and 10 control dogs were identified over an 8-year 
      period (1996-2004) in databases at Cornell University's College of Veterinary 
      Medicine. Case inclusion required serologic evidence of natural exposure to B. 
      burgdorferi and availability of renal tissue (frozen or paraffin embedded) 
      exhibiting pathology consistent with Lyme nephritis. METHODS: Renal tissue 
      samples were assessed using modified Steiner (silver) (MS) staining, 
      immunohistochemistry (IHC), polymerase chain reaction (PCR) using 4 primer sets 
      (eubacterial, B. burgdorferi, Bartonella, and canine genomic DNA), and 
      fluorescence in situ hybridization (FISH) using a 5'-cy3-eubacterial probe for 
      16S rRNA. RESULTS: MS stain was positive in 1 case; IHC was negative in all 
      cases. None of the B. burgdorferi or Bartonella PCR reactions was positive. Two 
      of the B. burgdorferi FISH analyses were positive. CONCLUSIONS AND CLINICAL 
      IMPORTANCE: Minimal evidence of the presence of intact B. burgdorferi or any 
      other bacterial organism was found in the renal tissue of dogs with suspected 
      Lyme nephritis. Direct renal invasion by B. burgdorferi organisms does not appear 
      to be responsible for this syndrome.
FAU - Hutton, T A
AU  - Hutton TA
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Cornell 
      University, Ithaca, NY, USA. tabitha@post.harvard.edu
FAU - Goldstein, R E
AU  - Goldstein RE
FAU - Njaa, B L
AU  - Njaa BL
FAU - Atwater, D Z
AU  - Atwater DZ
FAU - Chang, Y-F
AU  - Chang YF
FAU - Simpson, K W
AU  - Simpson KW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
SB  - IM
MH  - Animals
MH  - Borrelia burgdorferi/*isolation & purification
MH  - Dog Diseases/*microbiology
MH  - Dogs
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Kidney/microbiology
MH  - Kidney Diseases/microbiology/*veterinary
MH  - Lyme Disease/microbiology/*veterinary
MH  - Male
MH  - Polymerase Chain Reaction/veterinary
MH  - Staining and Labeling
EDAT- 2008/06/20 09:00
MHDA- 2008/10/29 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/10/29 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - JVIM131 [pii]
AID - 10.1111/j.1939-1676.2008.0131.x [doi]
PST - ppublish
SO  - J Vet Intern Med. 2008 Jul-Aug;22(4):860-5. doi: 10.1111/j.1939-1676.2008.0131.x.