PMID- 18564355
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20211203
IS  - 1365-2141 (Electronic)
IS  - 0007-1048 (Linking)
VI  - 142
IP  - 5
DP  - 2008 Sep
TI  - Multiplex ligation-dependent probe amplification for detection of genomic 
      alterations in chronic lymphocytic leukaemia.
PG  - 793-801
LID - 10.1111/j.1365-2141.2008.07268.x [doi]
AB  - Chronic lymphocytic leukaemia (CLL) is the commonest form of leukaemia in adults 
      in Western countries. We performed multiplex ligation-dependent probe 
      amplification (MLPA) analysis in 50 CLL patients to identify multiple genomic 
      CLL-specific targets, including genes located at 13q14, 17p13 (TP53), 11q23 (ATM) 
      and chromosome 12, and compared the results with those obtained with fluorescence 
      in situ hybridization (FISH). There was a good correlation between MLPA and FISH 
      results, as most alterations (89%) were detected by both techniques. Only three 
      cases with a low percentage (<25%) of cells carrying the alterations were not 
      detected by MLPA. On the other hand, as MLPA uses multiple probes it identified 
      intragenic or small alterations undetected by FISH in three cases. MLPA also 
      detected alterations in 8q24 (MYC) and 6q25-26. In summary, unlike interphase 
      FISH, MLPA enabled the simultaneous analysis of many samples with automated data 
      processing at a low cost. Therefore, the combination of robust multiplexing and 
      high throughput makes MLPA a useful technique for the analysis of genomic 
      alterations in CLL.
FAU - Coll-Mulet, Llorenc
AU  - Coll-Mulet L
AD  - Departament de Ciencies Fisiologiques II, IDIBELL-Universitat de Barcelona, 
      L'Hospitalet de Llobregat, Barcelona, Spain.
FAU - Santidrian, Antonio F
AU  - Santidrian AF
FAU - Cosialls, Ana M
AU  - Cosialls AM
FAU - Iglesias-Serret, Daniel
AU  - Iglesias-Serret D
FAU - de Frias, Merce
AU  - de Frias M
FAU - Grau, Javier
AU  - Grau J
FAU - Menoyo, Anna
AU  - Menoyo A
FAU - Gonzalez-Barca, Eva
AU  - Gonzalez-Barca E
FAU - Pons, Gabriel
AU  - Pons G
FAU - Domingo, Alicia
AU  - Domingo A
FAU - Gil, Joan
AU  - Gil J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080617
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.11.1 (ATM protein, human)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Ataxia Telangiectasia Mutated Proteins
MH  - Cell Cycle Proteins
MH  - Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 12
MH  - DNA-Binding Proteins
MH  - Gene Amplification
MH  - Gene Dosage
MH  - Genes, p53
MH  - Genomics/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Protein Serine-Threonine Kinases
MH  - Spain
MH  - Tumor Suppressor Proteins
EDAT- 2008/06/20 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/20 09:00
PHST- 2008/06/20 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/20 09:00 [entrez]
AID - BJH7268 [pii]
AID - 10.1111/j.1365-2141.2008.07268.x [doi]
PST - ppublish
SO  - Br J Haematol. 2008 Sep;142(5):793-801. doi: 10.1111/j.1365-2141.2008.07268.x. 
      Epub 2008 Jun 17.