PMID- 18565212
OWN - NLM
STAT- MEDLINE
DCOM- 20080826
LR  - 20211020
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 8
DP  - 2008 Jun 19
TI  - Human pregnane X receptor is expressed in breast carcinomas, potential 
      heterodimers formation between hPXR and RXR-alpha.
PG  - 174
LID - 10.1186/1471-2407-8-174 [doi]
AB  - BACKGROUND: The human pregnane X receptor (hPXR) is an orphan nuclear receptor 
      that induces transcription of response elements present in steroid-inducible 
      cytochrome P-450 gene promoters. This activation requires the participation of 
      retinoid X receptors (RXRs), needed partners of hPXR to form heterodimers. We 
      have investigated the expression of hPXR and RXRs in normal, premalignant, and 
      malignant breast tissues, in order to determine whether their expression profile 
      in localized infiltrative breast cancer is associated with an increased risk of 
      recurrent disease. METHODS: Breast samples from 99 patients including benign 
      breast diseases, in situ and infiltrative carcinomas were processed for 
      immunohistochemistry and Western-blot analysis. RESULTS: Cancer cells from 
      patients that developed recurrent disease showed a high cytoplasmic location of 
      both hPXR isoforms. Only the infiltrative carcinomas that relapsed before 48 
      months showed nuclear location of hPXR isoform 2. This location was associated 
      with the nuclear immunoexpression of RXR-alpha. CONCLUSION: Breast cancer cells 
      can express both variants 1 and 2 of hPXR. Infiltrative carcinomas that recurred 
      showed a nuclear location of both hPXR and RXR-alpha; therefore, the 
      overexpression and the subcellular location changes of hPXR could be considered 
      as a potential new prognostic indicator.
FAU - Conde, Isabel
AU  - Conde I
AD  - Department of Cell Biology and Genetics, University of Alcala, 28871 Alcala de 
      Henares, Madrid, Spain. iconde@cib.csic.es
FAU - Lobo, Maria V T
AU  - Lobo MV
FAU - Zamora, Javier
AU  - Zamora J
FAU - Perez, Julio
AU  - Perez J
FAU - Gonzalez, Francisco J
AU  - Gonzalez FJ
FAU - Alba, Emilio
AU  - Alba E
FAU - Fraile, Benito
AU  - Fraile B
FAU - Paniagua, Ricardo
AU  - Paniagua R
FAU - Arenas, Maria I
AU  - Arenas MI
LA  - eng
PT  - Journal Article
DEP - 20080619
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (Pregnane X Receptor)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Retinoid X Receptor alpha)
RN  - 0 (Retinoid X Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Breast Neoplasms/*metabolism/pathology/surgery
MH  - Carcinoma in Situ/metabolism/pathology/surgery
MH  - Carcinoma, Ductal, Breast/metabolism/pathology/surgery
MH  - Dimerization
MH  - Disease-Free Survival
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Pregnane X Receptor
MH  - Receptors, Steroid/*biosynthesis/metabolism
MH  - Retinoid X Receptor alpha/*metabolism
MH  - Retinoid X Receptors/*metabolism
PMC - PMC2442113
EDAT- 2008/06/21 09:00
MHDA- 2008/08/30 09:00
PMCR- 2008/06/19
CRDT- 2008/06/21 09:00
PHST- 2008/02/04 00:00 [received]
PHST- 2008/06/19 00:00 [accepted]
PHST- 2008/06/21 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/06/21 09:00 [entrez]
PHST- 2008/06/19 00:00 [pmc-release]
AID - 1471-2407-8-174 [pii]
AID - 10.1186/1471-2407-8-174 [doi]
PST - epublish
SO  - BMC Cancer. 2008 Jun 19;8:174. doi: 10.1186/1471-2407-8-174.