PMID- 18565501
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20231213
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 253
IP  - 1-2
DP  - 2008 May-Jun
TI  - Interactions between osteosarcoma cell lines and dendritic cells immune function: 
      An in vitro study.
PG  - 71-80
LID - 10.1016/j.cellimm.2008.05.002 [doi]
AB  - Dendritic cells (DCs) might be partly responsible for the defective immune 
      response in tumor bearing hosts, but no study in osteosarcoma patients is still 
      available. Therefore, we investigated in vitro whether human osteosarcoma cell 
      lines have an inhibitor effect on different types of DCs: CD14+DCs, DC1 and DC2. 
      DCs derived from healthy donors were cultured with osteosarcoma cell lines and 
      appropriate cytokine cocktails and analysed for the expression of co-stimulatory 
      molecules (CD40, CD80, CD83, CD86, HLA-DR). Each interaction resulted in a lower 
      phenotypic expression of the DCs maturation markers, especially on DC2. Moreover, 
      the addition of various cytokines and compounds (rhIL-12, CD40L, Indometacin) 
      induced the DC1 and DC2 subsets towards the Th1 pattern as shown by ELISA. 
      Osteosarcoma highly interferes with an in vitro DCs immune function as antigen 
      presenting cells. The understanding of tumor biology underlines the need for a 
      specific osteosarcoma immunotherapy able to reverse this immune-surveillance 
      inhibition.
FAU - Muraro, Michela
AU  - Muraro M
AD  - Stem Cell Transplantation and Cellular Therapy Unit, Pediatric Onco-Hematology 
      Department, Regina Margherita Children's Hospital, Piazza Polonia 94, 10126 
      Turin, Italy.
FAU - Mereuta, Oana M
AU  - Mereuta OM
FAU - Saglio, Francesco
AU  - Saglio F
FAU - Carraro, Francesca
AU  - Carraro F
FAU - Berger, Massimo
AU  - Berger M
FAU - Madon, Enrico
AU  - Madon E
FAU - Fagioli, Franca
AU  - Fagioli F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080618
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Biomarkers)
RN  - 0 (CD40 Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Membrane Glycoproteins)
RN  - 187348-17-0 (Interleukin-12)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Anti-Inflammatory Agents, Non-Steroidal/immunology
MH  - Antigens, CD/immunology
MH  - B7-1 Antigen/immunology
MH  - B7-2 Antigen/immunology
MH  - Biomarkers/metabolism
MH  - CD40 Antigens/immunology
MH  - *Cell Line, Tumor
MH  - Coculture Techniques
MH  - *Dendritic Cells/cytology/immunology
MH  - Humans
MH  - Immunity/*physiology
MH  - Immunoglobulins/immunology
MH  - Indomethacin/immunology
MH  - Interleukin-12/immunology
MH  - Lipopolysaccharide Receptors/immunology
MH  - Membrane Glycoproteins/immunology
MH  - *Osteosarcoma/immunology/pathology
MH  - Phenotype
MH  - CD83 Antigen
EDAT- 2008/06/21 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/06/21 09:00
PHST- 2008/01/03 00:00 [received]
PHST- 2008/04/24 00:00 [revised]
PHST- 2008/05/06 00:00 [accepted]
PHST- 2008/06/21 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/06/21 09:00 [entrez]
AID - S0008-8749(08)00086-5 [pii]
AID - 10.1016/j.cellimm.2008.05.002 [doi]
PST - ppublish
SO  - Cell Immunol. 2008 May-Jun;253(1-2):71-80. doi: 10.1016/j.cellimm.2008.05.002. 
      Epub 2008 Jun 18.