PMID- 1856592
OWN - NLM
STAT- MEDLINE
DCOM- 19910827
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 50
IP  - 3
DP  - 1991 Sep
TI  - Lipopolysaccharide-dependent and lipopolysaccharide-independent pathways of 
      monocyte desensitisation to lipopolysaccharides.
PG  - 215-22
AB  - The present study demonstrates that with time in culture blood monocytes (MO) 
      lose their ability to express procoagulant activity (PCA) and secrete tumor 
      necrosis factor-alpha (TNF alpha) in culture medium in response to 
      lipopolysaccharide (LPS) stimulation. Thus, upon 10 micrograms/ml LPS stimulation 
      for 4 hours 2-day-old MO produced lower levels of PCA and TNF alpha than fresh 
      MO. The decrease in responsiveness was not caused by cell death, since in the 
      case of TNF alpha it was fully reversible by interferon-gamma (IFN-gamma). 
      Compared with cells pre-incubated in medium alone, the responsiveness of MO 
      pre-incubated in LPS was further decreased. Thus, in MO LPS pre-incubation was 
      followed by an LPS refractory state. It was expected that the decrease in 
      responsiveness induced by cultivation in medium alone was mediated by LPS 
      contamination of culture medium. However, as we were unable to prevent this 
      decrease by neutralizing LPS contamination of the culture medium with polymyxin 
      B, the loss in LPS-induced activities of cultured MO is likely to be mediated by 
      culture conditions other than LPS contamination. Taken together the present data 
      demonstrate that LPS-dependent as well as LPS-independent pathways of MO 
      desensitization to LPS exist.
FAU - Annenkov, A Y
AU  - Annenkov AY
AD  - Laboratory of Transplantation Immunology, Institute of Transplantology and 
      Artificial Organs, Moscow, USSR.
FAU - Baranova, F S
AU  - Baranova FS
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Culture Media)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Culture Media
MH  - Drug Contamination
MH  - Humans
MH  - Lipopolysaccharides/blood/pharmacology/*physiology
MH  - Monocytes/*drug effects
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 10.1002/jlb.50.3.215 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1991 Sep;50(3):215-22. doi: 10.1002/jlb.50.3.215.