PMID- 18569602
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20121115
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 71
IP  - 13-14
DP  - 2008
TI  - Expression of N-acetyltransferase in monocyte-derived dendritic cells.
PG  - 960-4
LID - 10.1080/15287390801989135 [doi]
AB  - Dendritic cells (DCs) are known to internalize, process, and present 
      low-molecular-weight chemicals to T cells in the course of the sensitization and 
      elicitation phase of allergic contact dermatitis. Thus, DCs may be involved in 
      metabolic activation and detoxification of haptens and thereby influence the 
      quantity of immunogens inducing sensitization. Recently, the cytochrome P-450 
      enzymes expressed in monocyte-derived dendritic cells (MoDCs) were characterized. 
      In the present study, N-acetyltransferase 1 and 2 (NAT-1 and -2) mRNA expression 
      and N-acetylation capacities of these cells were investigated. Monocytes from 
      healthy donors were incubated with granulocyte-monocyte colony-stimulating factor 
      (GM-CSF) and interleukin (IL)-4 for 6 d and the resulting immature MoDCs were 
      characterized by flow cytometry. Total RNA from MoDCs was isolated, reverse 
      transcribed, and polymerase chain reaction (PCR) for NAT-1 and NAT-2 mRNA was 
      performed. Data showed the presence of mRNA for NAT-1 (9 of 10 donors) and NAT-2 
      (8 of 10 donors) in these cells. NAT-1 enzyme activities were achieved through 
      acetylation of para-aminobenzoic acid (PABA) by MoDC cell lysates and activities 
      varied between 23.4 and 26.6 nmol/mg/min. In addition, complete cell acetylation 
      of para-phenylenediamine (PPD), estimated via analysis of monoacetyl-PPD (MAPPD) 
      and diacetyl-PPD (DAPPD) in cell culture supernatants, confirmed that in vitro 
      generated MoDCs (4 of 6 donors) express metabolic active N-acetyltransferase 
      (NAT-1). In the case of PPD, our results emphasize that N-acetylation status may 
      influence the amounts of immunogens available for sensitization to PPD.
FAU - Lichter, Jutta
AU  - Lichter J
AD  - Department of Environmental Toxicology, Trier, Germany.
FAU - Heckelen, Angela
AU  - Heckelen A
FAU - Fischer, Klaus
AU  - Fischer K
FAU - Blomeke, Brunhilde
AU  - Blomeke B
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Isoenzymes)
RN  - 0 (Phenylenediamines)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.3.1.5 (Arylamine N-Acetyltransferase)
RN  - EC 2.3.1.5 (N-acetyltransferase 1)
RN  - EC 2.3.1.5 (NAT2 protein, human)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Acetylation
MH  - Arylamine N-Acetyltransferase/genetics/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*enzymology
MH  - Gene Expression Regulation, Enzymologic
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-4/pharmacology
MH  - Isoenzymes/genetics/*metabolism
MH  - Monocytes/*cytology/drug effects/enzymology
MH  - Phenylenediamines/metabolism
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2008/06/24 09:00
MHDA- 2008/07/23 09:00
CRDT- 2008/06/24 09:00
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - 793775809 [pii]
AID - 10.1080/15287390801989135 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2008;71(13-14):960-4. doi: 10.1080/15287390801989135.