PMID- 18570921
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20181201
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 314
IP  - 11-12
DP  - 2008 Jul 1
TI  - NRSF silencing induces neuronal differentiation of human mesenchymal stem cells.
PG  - 2257-65
LID - 10.1016/j.yexcr.2008.04.008 [doi]
AB  - Mesenchymal stem cells (MSCs) are multipotent cells that have the potential to 
      differentiate into the neuronal cell lineage. Here, we describe the highly 
      efficient and specific induction of cells with neuronal characteristics, without 
      glial differentiation, from human bone marrow-derived mesenchymal stem cells by 
      NRSF silencing. Cells that have the characteristics of MSCs were obtained from 
      human bone marrow. Lentiviral vectors were used to deliver small interference 
      NRSF RNA (siNRSF) into MSCs. After being infected with lentivirus containing 
      siNRSF, MSCs were successfully induced to differentiate into neuronal cells, 
      which exhibited neuron-like morphology and formed nissl bodies. These 
      differentiated cells expressed multiple neuron-specific genes including 
      brain-derived neurotrophic factor (BDNF), neurogenin 1 (NGN1), neuron-specific 
      enolase (NSE), synaptophysin (SYP), and neuron-specific growth-associated protein 
      (SCG10), as well as expressing mature neuronal marker proteins, such as 
      beta-tubulin III, NSE, microtubule-associated protein type 2 (MAP-2), and 
      neurofilament-200 (NF-200), yet did not express the glial markers glial 
      fibrillary acidic protein (GFAP) and oligodendrocyte transcription factor 2 
      (Olig2), as verified by immunofluorescence staining. The whole cell patch-clamp 
      technique recorded TTX-sensitive Na(+) currents and action potential from these 
      differentiated cells. Thus, our results demonstrate that NRSF silencing can 
      activate some neuronal genes and induce neuronal differentiation of mesenchymal 
      stem cells.
FAU - Yang, Yinxiang
AU  - Yang Y
AD  - Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion 
      Medicine, Beijing, China.
FAU - Li, Yanhua
AU  - Li Y
FAU - Lv, Yang
AU  - Lv Y
FAU - Zhang, Sainan
AU  - Zhang S
FAU - Chen, Lin
AU  - Chen L
FAU - Bai, Cixian
AU  - Bai C
FAU - Nan, Xue
AU  - Nan X
FAU - Yue, Wen
AU  - Yue W
FAU - Pei, Xuetao
AU  - Pei X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080501
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Biomarkers)
RN  - 0 (Ion Channels)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Cell Differentiation/*physiology
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Humans
MH  - Ion Channels/metabolism
MH  - Lentivirus/genetics/metabolism
MH  - Mesenchymal Stem Cells/cytology/*physiology
MH  - Neurons/cytology/*physiology
MH  - Patch-Clamp Techniques
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Repressor Proteins/genetics/*metabolism
EDAT- 2008/06/24 09:00
MHDA- 2008/07/23 09:00
CRDT- 2008/06/24 09:00
PHST- 2008/01/10 00:00 [received]
PHST- 2008/04/08 00:00 [revised]
PHST- 2008/04/16 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S0014-4827(08)00163-8 [pii]
AID - 10.1016/j.yexcr.2008.04.008 [doi]
PST - ppublish
SO  - Exp Cell Res. 2008 Jul 1;314(11-12):2257-65. doi: 10.1016/j.yexcr.2008.04.008. 
      Epub 2008 May 1.