PMID- 18571002
OWN - NLM
STAT- MEDLINE
DCOM- 20080815
LR  - 20171116
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 69
IP  - 6
DP  - 2008 Jun
TI  - Immunogenicity and immunomodulatory effects of amnion-derived multipotent 
      progenitor cells.
PG  - 321-8
LID - 10.1016/j.humimm.2008.04.007 [doi]
AB  - This is the first study on the immunologic properties of a clinically relevant 
      population of cells derived from the amnion of human placenta. Unlike other cells 
      from the amnion, these amnion-derived multipotent progenitor cells (AMP cells), 
      from human amnion, grow in serum-free conditions and have never been cultured in 
      the presence of medium containing animal-derived components. This study reports 
      the immunologic characteristics of AMP cells and their roles as immunomodulators. 
      Characterization of AMP cells revealed the presence of major histocompatibility 
      complex (MHC) class I but the lack of class II antigens and absence of 
      co-stimulatory molecules B7-1 and B7-2. The nonclassical human leukocyte antigen 
      (HLA)-G was expressed at low levels on cultured AMP cells. Expression was 
      significantly increased after interferon-gamma (IFN-gamma) treatment. Cultured 
      peripheral blood mononuclear cells did not respond to irradiated AMP cells, 
      indicated by lack of proliferation as measured by standard mixed lymphocyte 
      reaction. Culturing AMP cells with IFN-gamma did not reverse this result and did 
      not upregulate class II expression. The AMP cells were shown to have 
      immunomodulatory capabilities by inhibiting peripheral blood mononuclear cell 
      proliferative responses to mitogen, alloantigen, and recall antigen, but the AMP 
      cells were unable to inhibit preactivated T-cell blast response to growth factor 
      media. This immunomodulatory effect of AMP cells was found to be dependent on 
      cell-to-cell contact.
FAU - Banas, Richard Allan
AU  - Banas RA
AD  - Stemnion, Inc, Pittsburgh, PA, USA. rbanas@stemnion.com
FAU - Trumpower, Catherine
AU  - Trumpower C
FAU - Bentlejewski, Carol
AU  - Bentlejewski C
FAU - Marshall, Vivienne
AU  - Marshall V
FAU - Sing, George
AU  - Sing G
FAU - Zeevi, Adriana
AU  - Zeevi A
LA  - eng
PT  - Journal Article
DEP - 20080515
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amnion/*cytology/*immunology
MH  - B7-1 Antigen/immunology
MH  - B7-2 Antigen/immunology
MH  - Cell Adhesion/immunology
MH  - Cell Culture Techniques
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Epithelial Cells/cytology/immunology
MH  - Female
MH  - HLA Antigens/biosynthesis/immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Interferon-gamma/immunology
MH  - Lymphocyte Activation/immunology
MH  - Multipotent Stem Cells/*cytology/*immunology/metabolism
MH  - Placenta/cytology/immunology
MH  - Pregnancy
EDAT- 2008/06/24 09:00
MHDA- 2008/08/16 09:00
CRDT- 2008/06/24 09:00
PHST- 2008/04/02 00:00 [received]
PHST- 2008/04/05 00:00 [revised]
PHST- 2008/04/08 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/08/16 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S0198-8859(08)00068-2 [pii]
AID - 10.1016/j.humimm.2008.04.007 [doi]
PST - ppublish
SO  - Hum Immunol. 2008 Jun;69(6):321-8. doi: 10.1016/j.humimm.2008.04.007. Epub 2008 
      May 15.