PMID- 18571259
OWN - NLM
STAT- MEDLINE
DCOM- 20081021
LR  - 20191210
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 75
IP  - 1
DP  - 2008 Sep
TI  - In situ detection of bacteria in calcified biofilms using FISH and CARD-FISH.
PG  - 103-8
LID - 10.1016/j.mimet.2008.05.015 [doi]
AB  - Modified protocols of fluorescence in situ hybridization (FISH) and catalyze 
      reporter deposition fluorescence in situ hybridization (CARD-FISH) were developed 
      in order to detect bacteria in situ in calcified stromatolite biofilms. Smooth, 
      well-preserved thin sections of calcified biofilms (approximately 5 microm thin, 
      vertical sectioning of approximately 1 cm deep) were obtained by cryo-sectioning 
      using the adhesive tape-stabilization technique. A modified hybridization buffer 
      was applied during hybridization to prevent calcite dissolution as well as false 
      binding of oligonucleotide probes to the charged mineral surfaces. Particularly, 
      bright and specific CARD-FISH signals allowed the detection of bacteria in 
      intensively calcified biofilms even at low magnification, which is suitable for 
      investigating millimeter- to centimeter-scale vertical distribution patterns of 
      bacteria.
FAU - Shiraishi, Fumito
AU  - Shiraishi F
AD  - Geoscience Centre, University of Gottingen, Goldschmidtstrasse 3, D-37077 
      Gottingen, Germany. fshirai@gwdg.de
FAU - Zippel, Barbara
AU  - Zippel B
FAU - Neu, Thomas R
AU  - Neu TR
FAU - Arp, Gernot
AU  - Arp G
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080520
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Bacteria/*genetics/isolation & purification
MH  - *Bacteriological Techniques
MH  - *Biofilms
MH  - Fresh Water/microbiology
MH  - Geologic Sediments/*microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization/*methods
MH  - Oligonucleotide Probes/genetics
EDAT- 2008/06/24 09:00
MHDA- 2008/10/22 09:00
CRDT- 2008/06/24 09:00
PHST- 2008/03/27 00:00 [received]
PHST- 2008/05/02 00:00 [revised]
PHST- 2008/05/13 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/10/22 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S0167-7012(08)00195-4 [pii]
AID - 10.1016/j.mimet.2008.05.015 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2008 Sep;75(1):103-8. doi: 10.1016/j.mimet.2008.05.015. Epub 
      2008 May 20.