PMID- 18571880
OWN - NLM
STAT- MEDLINE
DCOM- 20081201
LR  - 20080901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 131
IP  - 3-4
DP  - 2008 Oct 15
TI  - Genetic variation in open reading frame 5 gene of porcine reproductive and 
      respiratory syndrome virus in Taiwan.
PG  - 339-47
LID - 10.1016/j.vetmic.2008.04.027 [doi]
AB  - In an effort to understand the genetic variation in porcine reproductive and 
      respiratory syndrome virus (PRRSV) isolates in Taiwan, 40 isolates obtained 
      between 2004 and 2006 were analyzed for their sequences of open reading frame 5. 
      After reverse transcription polymerase chain reaction, the amplified open reading 
      frame 5 fragments were analyzed by restriction fragment length polymorphism and 
      sequence comparison. The results showed that all the Taiwanese isolates belonged 
      to the North American genotype. Multiple patterns obtained from restriction 
      fragment length polymorphism, 83-99% nucleotide similarity and 84-99% deduced 
      amino acid similarity suggested a high level of genetic variation and PRRSV was 
      not a single invasion to Taiwan. Moreover, vaccine-like isolates were isolated 
      from the field, implying that some field isolates might originate from vaccine 
      virus.
FAU - Wang, Chun
AU  - Wang C
AD  - Division of Hog Cholera, Animal Health Research Institute, Council of 
      Agriculture, Executive Yuan, 376 Chung-Cheng Road, Tansui, Taipei County 251, 
      Taiwan. cwang@mail.nvri.gov.tw
FAU - Lee, Fan
AU  - Lee F
FAU - Huang, Tien-Shine
AU  - Huang TS
FAU - Pan, Chu-Hsiang
AU  - Pan CH
FAU - Jong, Ming-Hwa
AU  - Jong MH
FAU - Chao, Parn-Hwa
AU  - Chao PH
LA  - eng
PT  - Journal Article
DEP - 20080426
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Genetic Variation
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Swine
MH  - Taiwan/epidemiology
MH  - Viral Proteins/chemistry/genetics
EDAT- 2008/06/24 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/24 09:00
PHST- 2007/12/03 00:00 [received]
PHST- 2008/03/21 00:00 [revised]
PHST- 2008/04/10 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S0378-1135(08)00136-3 [pii]
AID - 10.1016/j.vetmic.2008.04.027 [doi]
PST - ppublish
SO  - Vet Microbiol. 2008 Oct 15;131(3-4):339-47. doi: 10.1016/j.vetmic.2008.04.027. 
      Epub 2008 Apr 26.