PMID- 18571954
OWN - NLM
STAT- MEDLINE
DCOM- 20090626
LR  - 20181201
IS  - 1532-2149 (Electronic)
IS  - 1090-3801 (Linking)
VI  - 13
IP  - 4
DP  - 2009 Apr
TI  - Facilitated neurotrophin release in sensitized human skin.
PG  - 399-405
LID - 10.1016/j.ejpain.2008.05.005 [doi]
AB  - The neurotrophins nerve growth factor (NGF) and brain-derived neurotrophic factor 
      (BDNF) play a pivotal role in the generation and maintenance of hyperalgesia. In 
      the present study we analyzed NGF and BDNF levels in human skin of the upper arm 
      and axilla skin sites by dermal microdialysis and multiplexed assay. Skin 
      sensitization and inflammatory responses were evoked experimentally by repetitive 
      shaving of one axilla provoking local erythema and reduced heat pain thresholds. 
      Acute excitation of skin nociceptors was performed by perfusion of the 
      microdialysis catheters with citric acid pH 3. At baseline, neurotrophin 
      concentrations did not differ significantly between the investigated skin sites. 
      On average, NGF concentration was 5.8 fg/microug protein/ml sample volume and 
      BDNF concentration was 87.5 fg/microg/ml. Citric acid perfusion marginally 
      increased NGF levels to 7.3 fg/microg/ml on average in the upper arm and control 
      axilla. Similarly, BDNF values increased at these control skin sites to about 122 
      fg/microg/ml following proton stimulation. In contrast, perfusion of the inflamed 
      axilla with citric acid significantly enhanced the release of both NGF and BDNF. 
      On average, NGF levels were analyzed at 14.6 fg/microg/ml and BDNF values at 202 
      fg/microg/ml. These data demonstrate enhanced level of neurotrophin release in 
      inflamed human skin in vivo which might well contribute to peripheral 
      sensitization. Analyses of neurotrophic factors by dermal microdialysis are 
      useful endogenous markers to further explore their role in neuronal sensitization 
      processes in human.
FAU - Paterson, Sarah
AU  - Paterson S
AD  - Unilever R&D Laboratory Port Sunlight, Bebington, Wirral, United Kingdom.
FAU - Schmelz, Martin
AU  - Schmelz M
FAU - McGlone, Francis
AU  - McGlone F
FAU - Turner, Graham
AU  - Turner G
FAU - Rukwied, Roman
AU  - Rukwied R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080620
PL  - England
TA  - Eur J Pain
JT  - European journal of pain (London, England)
JID - 9801774
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Nerve Growth Factors)
RN  - 2968PHW8QP (Citric Acid)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Adult
MH  - Biomarkers/analysis/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Citric Acid/pharmacology
MH  - Dermatitis/*metabolism/physiopathology
MH  - Erythema/metabolism/physiopathology
MH  - Female
MH  - Humans
MH  - Hyperalgesia/*metabolism/physiopathology
MH  - Inflammation Mediators/pharmacology
MH  - Microdialysis
MH  - Middle Aged
MH  - Nerve Growth Factor/metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Pain Measurement
MH  - Pain Threshold/physiology
MH  - Sensory Receptor Cells/*metabolism
MH  - Skin/innervation/*metabolism/physiopathology
MH  - Up-Regulation/physiology
EDAT- 2008/06/24 09:00
MHDA- 2009/06/27 09:00
CRDT- 2008/06/24 09:00
PHST- 2008/01/16 00:00 [received]
PHST- 2008/04/25 00:00 [revised]
PHST- 2008/05/11 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2009/06/27 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S1090-3801(08)00110-9 [pii]
AID - 10.1016/j.ejpain.2008.05.005 [doi]
PST - ppublish
SO  - Eur J Pain. 2009 Apr;13(4):399-405. doi: 10.1016/j.ejpain.2008.05.005. Epub 2008 
      Jun 20.