PMID- 18572188
OWN - NLM
STAT- MEDLINE
DCOM- 20080912
LR  - 20080728
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 337
IP  - 1
DP  - 2008 Aug 20
TI  - Optimizing ELISAs for precision and robustness using laboratory automation and 
      statistical design of experiments.
PG  - 35-41
LID - 10.1016/j.jim.2008.05.012 [doi]
AB  - Transferring manual ELISAs to automated platforms requires optimizing the assays 
      for each particular robotic platform. These optimization experiments are often 
      time consuming and difficult to perform using a traditional one-factor-at-a-time 
      strategy. In this manuscript we describe the development of an automated process 
      using statistical design of experiments (DOE) to quickly optimize immunoassays 
      for precision and robustness on the Tecan EVO liquid handler. By using fractional 
      factorials and a split-plot design, five incubation time variables and four 
      reagent concentration variables can be optimized in a short period of time.
FAU - Joelsson, Daniel
AU  - Joelsson D
AD  - Bioprocess & Bioanalytical Research, Merck & Co. Inc., West Point, PA 19486, USA. 
      daniel_joelsson@merck.com
FAU - Moravec, Phil
AU  - Moravec P
FAU - Troutman, Matthew
AU  - Troutman M
FAU - Pigeon, Joseph
AU  - Pigeon J
FAU - DePhillips, Pete
AU  - DePhillips P
LA  - eng
PT  - Journal Article
DEP - 20080612
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
SB  - IM
MH  - Animals
MH  - Automation/*statistics & numerical data
MH  - Data Interpretation, Statistical
MH  - Enzyme-Linked Immunosorbent Assay/*standards/*statistics & numerical data
MH  - Factor Analysis, Statistical
MH  - Humans
MH  - *Models, Statistical
MH  - Reproducibility of Results
MH  - Research Design/*statistics & numerical data
MH  - Robotics
MH  - Time Factors
EDAT- 2008/06/24 09:00
MHDA- 2008/09/16 09:00
CRDT- 2008/06/24 09:00
PHST- 2007/11/05 00:00 [received]
PHST- 2008/05/08 00:00 [revised]
PHST- 2008/05/13 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/09/16 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S0022-1759(08)00187-7 [pii]
AID - 10.1016/j.jim.2008.05.012 [doi]
PST - ppublish
SO  - J Immunol Methods. 2008 Aug 20;337(1):35-41. doi: 10.1016/j.jim.2008.05.012. Epub 
      2008 Jun 12.