PMID- 18572269
OWN - NLM
STAT- MEDLINE
DCOM- 20081008
LR  - 20181201
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Linking)
VI  - 129
IP  - 3
DP  - 2008 Aug 7
TI  - Efficient gene delivery in human and rodent mast cells using adenovirus vectors.
PG  - 215-22
LID - 10.1016/j.jconrel.2008.04.023 [doi]
AB  - Mast cells (MCs) have been shown to play an important role in immunoglobulin E 
      (IgE)-associated immediate hypersensitivity and innate immunity by producing a 
      variety of lipid mediators and cytokines. An efficient gene-delivery system is 
      indispensable for elucidation of these mechanisms. In the present study, human 
      and rodent MCs were transduced with various types of modified adenovirus (Ad) 
      vectors. Fiber modification in Ad vectors significantly improved the transduction 
      efficiencies in MCs. A fiber-substituted Ad serotype 5 (Ad5) vector containing Ad 
      serotype 35 (Ad35) fiber proteins (Ad5F35) and an Ad35 vector, both of which 
      transduce cells via human CD46, mediated 9.9-fold and 10.1-fold higher 
      transduction efficiencies than conventional Ad5 vectors in the human mast cell 
      line LAD2 among the Ad vectors. Ad5F35 and Ad35 vectors also efficiently 
      transduced bone marrow-derived MCs (BMMCs) prepared from human CD46-transgenic 
      (CD46TG) mice. The rat mast cell line RBL-2H3 were most efficiently transduced 
      with a fiber-mutant Ad5 vector containing the Arg-Gly-Asp (RGD) peptide in the HI 
      loop (Ad-RGD) of the fiber knob. Transduction with the Ad vectors did not induce 
      degranulation or inflammatory cytokine production in the MCs. These results 
      indicate that Ad vectors, including fiber-mutant Ad vectors, are effective 
      gene-delivery tools for MCs.
FAU - Nakashima, Kazuko
AU  - Nakashima K
AD  - Laboratory of Gene Transfer and Regulation, National Institute of Biomedical 
      Innovation, Osaka, Japan.
FAU - Sakurai, Fuminori
AU  - Sakurai F
FAU - Kawabata, Kenji
AU  - Kawabata K
FAU - Mizuguchi, Hiroyuki
AU  - Mizuguchi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080506
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Oligopeptides)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Line
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors/administration & 
      dosage/chemistry/classification/*genetics/metabolism
MH  - Humans
MH  - Mast Cells/cytology/*metabolism
MH  - Membrane Cofactor Protein/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Oligopeptides/chemistry
MH  - Protein Structure, Secondary
MH  - Transduction, Genetic/*methods
MH  - Transgenes
MH  - beta-N-Acetylhexosaminidases/analysis/metabolism
EDAT- 2008/06/24 09:00
MHDA- 2008/10/09 09:00
CRDT- 2008/06/24 09:00
PHST- 2008/01/22 00:00 [received]
PHST- 2008/04/28 00:00 [revised]
PHST- 2008/04/28 00:00 [accepted]
PHST- 2008/06/24 09:00 [pubmed]
PHST- 2008/10/09 09:00 [medline]
PHST- 2008/06/24 09:00 [entrez]
AID - S0168-3659(08)00245-9 [pii]
AID - 10.1016/j.jconrel.2008.04.023 [doi]
PST - ppublish
SO  - J Control Release. 2008 Aug 7;129(3):215-22. doi: 10.1016/j.jconrel.2008.04.023. 
      Epub 2008 May 6.