PMID- 18573344
OWN - NLM
STAT- MEDLINE
DCOM- 20081029
LR  - 20211203
IS  - 1095-9327 (Electronic)
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 39
IP  - 1
DP  - 2008 Sep
TI  - A bi-directional carboxypeptidase E-driven transport mechanism controls BDNF 
      vesicle homeostasis in hippocampal neurons.
PG  - 63-73
LID - 10.1016/j.mcn.2008.05.016 [doi]
AB  - Anterograde transport of brain-derived neurotrophic factor (BDNF) vesicles from 
      the soma to neurite terminals is necessary for activity-dependent secretion of 
      BDNF to mediate synaptic plasticity, memory and learning, and retrograde BDNF 
      transport back to the soma for recycling. In our study, overexpression of the 
      cytoplasmic tail of the carboxypeptidase E (CPE) found in BDNF vesicles 
      significantly reduced localization of BDNF in neurites of hippocampal neurons. 
      Live-cell imaging showed that the velocity and distance of movement of 
      fluorescent protein-tagged CPE- or BDNF-containing vesicles were reduced in both 
      directions. In pulldown assays, the CPE tail interacted with dynactin along with 
      kinesin-2 and kinesin-3, and cytoplasmic dynein. Competition assays using a CPE 
      tail peptide verified specific interaction between the CPE tail and dynactin. 
      Thus, the CPE cytoplasmic tail binds dynactin that recruits kinesins or dynein 
      for driving bi-directional transport of BDNF vesicle to maintain vesicle 
      homeostasis and secretion in hippocampal neurons.
FAU - Park, Joshua J
AU  - Park JJ
AD  - Section on Cellular Neurobiology, Developmental Neurobiology Program, National 
      Institute of Child Health and Human Development, National Institutes of Health, 
      Bethesda, MD, 20892, USA.
FAU - Cawley, Niamh X
AU  - Cawley NX
FAU - Loh, Y Peng
AU  - Loh YP
LA  - eng
GR  - Z01 HD000056-32/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20080604
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.4.17.10 (Carboxypeptidase H)
RN  - EC 3.6.4.2 (Dyneins)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Carboxypeptidase H/chemistry/genetics/*metabolism
MH  - Dyneins/genetics/metabolism
MH  - Hippocampus/*cytology/metabolism
MH  - Homeostasis
MH  - Kinesins/genetics/metabolism
MH  - Mice
MH  - Neurons/cytology/*metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Synaptic Vesicles/*metabolism
PMC - PMC2606928
MID - NIHMS68985
EDAT- 2008/06/25 09:00
MHDA- 2008/10/31 09:00
PMCR- 2009/09/01
CRDT- 2008/06/25 09:00
PHST- 2008/03/14 00:00 [received]
PHST- 2008/05/12 00:00 [revised]
PHST- 2008/05/22 00:00 [accepted]
PHST- 2008/06/25 09:00 [pubmed]
PHST- 2008/10/31 09:00 [medline]
PHST- 2008/06/25 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - S1044-7431(08)00138-3 [pii]
AID - 10.1016/j.mcn.2008.05.016 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2008 Sep;39(1):63-73. doi: 10.1016/j.mcn.2008.05.016. Epub 
      2008 Jun 4.