PMID- 18573563
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20080812
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Linking)
VI  - 130
IP  - 1
DP  - 2008 Aug 25
TI  - Gene delivery to dendritic cells mediated by complexes of lipoplexes and 
      pH-sensitive fusogenic polymer-modified liposomes.
PG  - 77-83
LID - 10.1016/j.jconrel.2008.05.007 [doi]
AB  - Dendritic cells (DCs) are potent professional antigen presenting cells that are 
      useful for cancer immunotherapy. We previously reported the preparation and 
      characterization of complexes of lipoplexes with pH-sensitive fusogenic 
      liposomes, which comprise polymers based on poly(glycidol) with carboxyl groups, 
      to transfect various malignant cell lines. The present study applied this kind of 
      vectors to transfection of a murine DC line DC2.4. We first optimized the ratios 
      of their components for efficient transfection. We subsequently investigated the 
      effects of ligands and pH-sensitive polymers to improve transfection activities. 
      Our results indicate that the anionic surface derived from pH-sensitive polymers 
      might be recognized by scavenger receptors on DC2.4 cells. In addition, no 
      effects on transfection or cell association were observed by attaching ligands 
      such as transferrin and mannan. We found that more sensitive pH-responding 
      polymers led to higher transfection activities into DC2.4 cells, which suggest 
      that endosomal escape is important for transfection into DC2.4 cells. These 
      complexes with pH-sensitive fusogenic polymers exhibited higher transfection 
      activity toward DC2.4 cells than some commercial reagents and hence may be useful 
      as a gene vector for DCs.
FAU - Yuba, Eiji
AU  - Yuba E
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture 
      University, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka 599-8531, Japan.
FAU - Kojima, Chie
AU  - Kojima C
FAU - Sakaguchi, Naoki
AU  - Sakaguchi N
FAU - Harada, Atsushi
AU  - Harada A
FAU - Koiwai, Kazunori
AU  - Koiwai K
FAU - Kono, Kenji
AU  - Kono K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080516
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (3,5-dipentadecyloxybenzamidine hydrochloride)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Benzamidines)
RN  - 0 (Fatty Acids)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Liposomes)
RN  - 0 (Polymers)
RN  - 0 (Propylene Glycols)
RN  - 0 (polyglycidol)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/administration & dosage/genetics
MH  - Benzamidines/chemistry
MH  - Cell Line
MH  - Dendritic Cells/*metabolism
MH  - Fatty Acids/chemistry
MH  - *Gene Transfer Techniques
MH  - Green Fluorescent Proteins/genetics
MH  - Histocompatibility Antigens Class I/genetics
MH  - Hydrogen-Ion Concentration
MH  - Liposomes
MH  - Luciferases/genetics
MH  - Mice
MH  - Molecular Structure
MH  - Polymers/*chemistry
MH  - Propylene Glycols/chemistry
MH  - Transfection
EDAT- 2008/06/25 09:00
MHDA- 2008/11/04 09:00
CRDT- 2008/06/25 09:00
PHST- 2007/09/11 00:00 [received]
PHST- 2008/05/02 00:00 [revised]
PHST- 2008/05/03 00:00 [accepted]
PHST- 2008/06/25 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/06/25 09:00 [entrez]
AID - S0168-3659(08)00249-6 [pii]
AID - 10.1016/j.jconrel.2008.05.007 [doi]
PST - ppublish
SO  - J Control Release. 2008 Aug 25;130(1):77-83. doi: 10.1016/j.jconrel.2008.05.007. 
      Epub 2008 May 16.