PMID- 18574593
OWN - NLM
STAT- MEDLINE
DCOM- 20080919
LR  - 20211020
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 453
IP  - 1
DP  - 2008 Jul
TI  - Comparison of different techniques for the detection of genetic risk-identifying 
      chromosomal gains and losses in neuroblastoma.
PG  - 47-55
LID - 10.1007/s00428-008-0633-6 [doi]
AB  - Neuroblastoma (NB) is a pediatric neoplasia that shows complex combinations of 
      acquired genetic aberrations. The specific genes and the molecular mechanisms 
      responsible for development and progression of NB remain poorly understood. Our 
      main objective is to compare the results obtained with different techniques for 
      the detection of genomic data in 20 patients with NB using the information 
      obtained to select the appropriate technique in routine analysis for the 
      therapeutic stratification. The genetic methods used in this study are multiprobe 
      fluorescence in situ hybridization (FISH) assay, metaphasic comparative genomic 
      hybridization (mCGH), array comparative genomic hybridization (aCGH), and the 
      multiplex ligation-dependent probe amplification (MLPA). Genomic copy number 
      abnormalities were used to group the cases in four categories: MYCN amplification 
      cases; 11q deletion tumors; cases with partial chromosome gains or losses and 
      samples with entire chromosome alterations. The data obtained from the 
      multigenomic techniques showed a high degree of concordance and our findings 
      support the hypothesis that NB consists of biologically distinct subgroups that 
      differ by genetic characteristics of prognostic relevance. FISH will be essential 
      for the mandatory study of MYCN status. The use of MLPA as routine technique is 
      an advantage procedure for detecting the implication of the common genetic 
      alterations in NB.
FAU - Villamon, Eva
AU  - Villamon E
AD  - Department of Pathology, Medical School, University of Valencia, Avda/Blasco 
      Ibanez, 17, Valencia, Spain.
FAU - Piqueras, Marta
AU  - Piqueras M
FAU - Mackintosh, Carlos
AU  - Mackintosh C
FAU - Alonso, Javier
AU  - Alonso J
FAU - de Alava, Enrique
AU  - de Alava E
FAU - Navarro, Samuel
AU  - Navarro S
FAU - Noguera, Rosa
AU  - Noguera R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080624
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (DNA, Neoplasm)
RN  - 0 (MYCN protein, human)
RN  - 0 (N-Myc Proto-Oncogene Protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins)
SB  - IM
MH  - Biopsy
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - DNA, Neoplasm/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Infant, Newborn
MH  - N-Myc Proto-Oncogene Protein
MH  - Neuroblastoma/diagnosis/*genetics/pathology
MH  - Nuclear Proteins/genetics
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Nucleic Acid Hybridization/*methods
MH  - Oncogene Proteins/genetics
MH  - Prognosis
EDAT- 2008/06/25 09:00
MHDA- 2008/09/20 09:00
CRDT- 2008/06/25 09:00
PHST- 2008/02/04 00:00 [received]
PHST- 2008/05/13 00:00 [accepted]
PHST- 2008/05/06 00:00 [revised]
PHST- 2008/06/25 09:00 [pubmed]
PHST- 2008/09/20 09:00 [medline]
PHST- 2008/06/25 09:00 [entrez]
AID - 10.1007/s00428-008-0633-6 [doi]
PST - ppublish
SO  - Virchows Arch. 2008 Jul;453(1):47-55. doi: 10.1007/s00428-008-0633-6. Epub 2008 
      Jun 24.