PMID- 18575864 OWN - NLM STAT- MEDLINE DCOM- 20081015 LR - 20171116 IS - 0939-5555 (Print) IS - 0939-5555 (Linking) VI - 87 IP - 10 DP - 2008 Oct TI - Contribution of neural cell adhesion molecule (NCAM) to hemopoietic system in monkeys. PG - 797-807 LID - 10.1007/s00277-008-0513-9 [doi] AB - Neural cell adhesion molecules (CD56) are important adhesion molecules that are mainly expressed on neural cells and natural killer cells. Although freshly isolated cynomolgus monkey bone marrow cells (BMCs) contained only a few CD56-positive cells, almost all the BM adherent cells (obtained after a 2- to 3-week culture of the BMCs) were stained positively with anti-CD56 monoclonal antibody (mAb). The BM adherent cells showed uniformly fibroblastic morphology and were negative for hematolymphoid markers (CD4, CD8, CD11b, CD14, CD34, and CD45). Adipogenesis and osteogenesis were observed under inductive culture conditions. The BM adherent cells had the ability to support hemopoiesis of hemopoietic stem cells (HSCs) in vitro, and the proliferation of HSCs was significantly inhibited by the addition of anti-CD56 mAb to the coculture system. CD56 molecules were also expressed on HSCs because about 20% of an HSC-enriched population (lineage-negative and blast-gated cells) was positive for CD56. In addition, the immunostaining of monkey BM sections revealed that many stromal cells were CD56-positive, and some CD56-positive stromal cells came into direct contact with CD56-positive hemopoietic cells. These results indicate that the CD56 molecule is expressed on both HSCs and BM stromal cells (containing MSCs) in monkeys, and therefore it can be speculated that CD56 also contributes to the human hematopoietic system. FAU - Kato, Junko AU - Kato J AD - 1st Department of Pathology, Kansai Medical University, Moriguchi, Osaka, Japan. FAU - Hisha, Hiroko AU - Hisha H FAU - Wang, Xiao-Li AU - Wang XL FAU - Mizokami, Tomomi AU - Mizokami T FAU - Okazaki, Satoshi AU - Okazaki S FAU - Li, Qing AU - Li Q FAU - Song, Chang-Ye AU - Song CY FAU - Maki, Masahiko AU - Maki M FAU - Hosaka, Naoki AU - Hosaka N FAU - Adachi, Yasushi AU - Adachi Y FAU - Inaba, Muneo AU - Inaba M FAU - Ikehara, Susumu AU - Ikehara S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080625 PL - Germany TA - Ann Hematol JT - Annals of hematology JID - 9107334 RN - 0 (CD56 Antigen) RN - 0 (Neural Cell Adhesion Molecules) SB - IM MH - Adipocytes/chemistry/physiology MH - Animals MH - Bone Marrow Cells/cytology/metabolism MH - CD56 Antigen/metabolism MH - Cell Adhesion/physiology MH - Cell Differentiation MH - Cells, Cultured MH - Hematopoietic System/*physiology MH - Humans MH - Immunophenotyping MH - *Macaca fascicularis MH - Neural Cell Adhesion Molecules/genetics/*metabolism MH - Osteoblasts/cytology/physiology MH - Stromal Cells/cytology/metabolism EDAT- 2008/06/26 09:00 MHDA- 2008/10/16 09:00 CRDT- 2008/06/26 09:00 PHST- 2007/09/04 00:00 [received] PHST- 2008/05/21 00:00 [accepted] PHST- 2008/06/26 09:00 [pubmed] PHST- 2008/10/16 09:00 [medline] PHST- 2008/06/26 09:00 [entrez] AID - 10.1007/s00277-008-0513-9 [doi] PST - ppublish SO - Ann Hematol. 2008 Oct;87(10):797-807. doi: 10.1007/s00277-008-0513-9. Epub 2008 Jun 25.