PMID- 18576086
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20080908
LR  - 20080625
IS  - 0273-2289 (Print)
IS  - 0273-2289 (Linking)
VI  - 63-65
DP  - 1997 Spring
TI  - Stabilization and reutilization of Bacillus megaterium glucose dehydrogenase by 
      immobilization.
PG  - 257-68
AB  - Glucose dehydrogenase (GDH) from Bacillus megaterium was immobilized using 
      aminopropyl controlled-pore silica (CPS, average pore sizes of 170 and 500 A) as 
      a support and glutaraldehyde as a bifunctional crosslinking agent. The 
      CPS-immobilized enzyme could be reused 12 times and the best results were 
      obtained using aminopropyl CPS-500 and bovine serum albumin as a feeder for 
      stabilizing the protein layer on the support. DEAE-Sephadex (A-25 and A-50) was 
      also used as a support for immobilizing GDH, with yields of around 42% for A-25 
      and 25-30% for A-50. The effect of pH on the immobilization procedure showed pH 
      6.5 to be better than pH 7.5 with respect to the recovery of enzyme activity. 
      Both preparations of DEAE-Sephadex immobilized GDH could be reused several times 
      and were thermostable at 40 degrees C for 7 h. The kinetic parameters as 
      Michaelis constant and maximum rate were determined for the immobilized enzyme 
      and compared with those for the freeform.
FAU - Baron, M
AU  - Baron M
AD  - Biomass Chemo/Biotechnology Laboratory, Department of Biochemistry, Federal 
      University of Parana, P. O. Box 19046 Curitiba , PR (81531-970), Brazil.
FAU - Fontana, J D
AU  - Fontana JD
FAU - Guimaraes, M F
AU  - Guimaraes MF
FAU - Woodward, J
AU  - Woodward J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1007/BF02920429 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 1997 Spring;63-65:257-68. doi: 10.1007/BF02920429.