PMID- 18576699
OWN - NLM
STAT- MEDLINE
DCOM- 20081016
LR  - 20081008
IS  - 0022-3395 (Print)
IS  - 0022-3395 (Linking)
VI  - 94
IP  - 4
DP  - 2008 Aug
TI  - Physical localization of the retrotransposons Boudicca and Perere 03 in 
      Schistosoma mansoni.
PG  - 993-5
LID - 10.1645/GE-1167.1 [doi]
AB  - Schistosoma mansoni is 1 of the causative agents of schistosomiasis, an endemic 
      disease in 76 countries of the world. The study of its genome, estimated to be 
      270 Mb, is very important to understanding schistosome biology, the mechanisms of 
      drug resistance, and immune evasion. Repetitive elements constitute more than 40% 
      of the S. mansoni genome and may play a role in the parasite evolution. The 
      retrotransposons Boudicca, a long terminal repeat (LTR), and Perere 03, a 
      non-LTR, are present in a high number in the S. mansoni genome and were localized 
      with the use of fluorescence in situ hybridization (FISH) and primed in situ 
      labeling (PRINS). Bacterial artificial chromosomes (BAC) clones containing the 
      retrotransposons Boudicca and Perere 03 were selected by bioinformatic analysis 
      and used as probes in FISH. Using metaphase chromosomes from sporocysts and the 
      FISH and PRINS techniques, we were able to map these retrotransposons. Perere 03 
      was localized in the euchromatic regions of the short arm of chromosome 2 and 
      Boudicca in the euchromatic regions of the short arm of chromosomes 2 and Z.
FAU - Valentim, C L L
AU  - Valentim CL
AD  - Departamento de Ciencias Biologicas e Nucleo de Pesquisas em Ciencias Biologicas 
      (NUPEB) Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
FAU - Gomes, M S
AU  - Gomes MS
FAU - Jeremias, W J
AU  - Jeremias WJ
FAU - Cunha, J C
AU  - Cunha JC
FAU - Oliveira, G C
AU  - Oliveira GC
FAU - Botelho, A C C
AU  - Botelho AC
FAU - Pimenta, P F P
AU  - Pimenta PF
FAU - Janotti-Passos, L K
AU  - Janotti-Passos LK
FAU - Guerra-Sa, R
AU  - Guerra-Sa R
FAU - Baba, E H
AU  - Baba EH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
RN  - 0 (Retroelements)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Genome, Helminth/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Microscopy, Confocal
MH  - Primed In Situ Labeling
MH  - Retroelements/*physiology
MH  - Schistosoma mansoni/*genetics
MH  - Sequence Alignment
MH  - Terminal Repeat Sequences
EDAT- 2008/06/26 09:00
MHDA- 2008/10/17 09:00
CRDT- 2008/06/26 09:00
PHST- 2006/12/11 00:00 [received]
PHST- 2007/12/20 00:00 [accepted]
PHST- 2008/06/26 09:00 [pubmed]
PHST- 2008/10/17 09:00 [medline]
PHST- 2008/06/26 09:00 [entrez]
AID - GE-1167 [pii]
AID - 10.1645/GE-1167.1 [doi]
PST - ppublish
SO  - J Parasitol. 2008 Aug;94(4):993-5. doi: 10.1645/GE-1167.1.