PMID- 18579298 OWN - NLM STAT- MEDLINE DCOM- 20081105 LR - 20220310 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 440 IP - 3 DP - 2008 Aug 8 TI - BDNF improves the effects of neural stem cells on the rat model of Alzheimer's disease with unilateral lesion of fimbria-fornix. PG - 331-5 LID - 10.1016/j.neulet.2008.05.107 [doi] AB - In this study, neural stem cells (NSCs) were obtained from the hippocampus using the serum-free culturing. NSCs labeled with 5'-bromo-2'-deoxyuridine (BrdU) were transplanted into transected rat basal forebrain followed by the injection of brain-derived neurotrophic factor (BDNF) into the lateral ventricle. Nestin staining and double-labeling immunohistochemistry were used to detect cell survival and neuronal differentiation of the BrdU labeled cells in the basal forebrain and it was observed that labeled NSCs differentiated into neurons and astrocytes in the basal forebrain. Immunohistochemical detection of p75(NGFR) indicated that the number of cholinergic neurons of the combination groups treated by NSCs, BDNF, and NSCs groups had more significant improvement than that of the injured groups in medial septum (MS) and vertical diagonal branch (VDB). Learning and memory abilities were also measured by Y-maze test and the results support that BDNF can enhance the treatment effects of NSCs transplanted into brain lesion model. FAU - Xuan, A G AU - Xuan AG AD - Department of Anatomy, Guangzhou Medical College, 195 Dongfeng Xi Road, Guangzhou 510182, PR China. FAU - Long, D H AU - Long DH FAU - Gu, H G AU - Gu HG FAU - Yang, D D AU - Yang DD FAU - Hong, L P AU - Hong LP FAU - Leng, S L AU - Leng SL LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080605 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Tissue Proteins) RN - 0 (Receptor, Nerve Growth Factor) RN - G34N38R2N1 (Bromodeoxyuridine) SB - IM MH - Alzheimer Disease/complications/*pathology/*therapy MH - Animals MH - Animals, Newborn MH - Behavior, Animal MH - Brain-Derived Neurotrophic Factor/*therapeutic use MH - Bromodeoxyuridine/metabolism MH - Cell Differentiation/drug effects MH - Disease Models, Animal MH - Fornix, Brain/drug effects/*injuries MH - Functional Laterality MH - Hippocampus/cytology MH - Male MH - Maze Learning/drug effects/physiology MH - Nerve Tissue Proteins/metabolism MH - Neurons/*drug effects/physiology MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, Nerve Growth Factor/metabolism MH - Stem Cell Transplantation/methods MH - Stem Cells/*drug effects/physiology EDAT- 2008/06/27 09:00 MHDA- 2008/11/06 09:00 CRDT- 2008/06/27 09:00 PHST- 2008/03/25 00:00 [received] PHST- 2008/05/21 00:00 [revised] PHST- 2008/05/24 00:00 [accepted] PHST- 2008/06/27 09:00 [pubmed] PHST- 2008/11/06 09:00 [medline] PHST- 2008/06/27 09:00 [entrez] AID - S0304-3940(08)00753-2 [pii] AID - 10.1016/j.neulet.2008.05.107 [doi] PST - ppublish SO - Neurosci Lett. 2008 Aug 8;440(3):331-5. doi: 10.1016/j.neulet.2008.05.107. Epub 2008 Jun 5.