PMID- 18579793
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 112
IP  - 5
DP  - 2008 Sep 1
TI  - Human natural killer cells exposed to IL-2, IL-12, IL-18, or IL-4 differently 
      modulate priming of naive T cells by monocyte-derived dendritic cells.
PG  - 1776-83
LID - 10.1182/blood-2008-02-135871 [doi]
AB  - Dendritic cells (DCs) play a crucial role in naive T-cell priming. Recent data 
      suggested that natural killer (NK) cells can influence the capability of DCs to 
      promote Th1 polarization. This regulatory function is primarily mediated by 
      cytokines released in the microenvironment during inflammatory responses 
      involving NK cells. In this study, we show that human NK cells exposed for short 
      time to interleukin (IL)-12, IL-2, or IL-18, promote distinct pathways of Th1 
      priming. IL-12- or IL-2-conditioned NK cells induce maturation of DCs capable of 
      priming IFN-gamma-producing Th1 cells. On the other hand, IL-18-conditioned NK 
      cells induce Th1 polarization only when cocultured with both DCs and T cells. In 
      this case, IL-2 released by T cells and IL-12 derived from DCs during the priming 
      process promote interferon (IFN)-gamma production. In contrast, when NK cells are 
      exposed to IL-4, nonpolarized T cells releasing only low levels of IL-2 are 
      generated. Thus, the prevalence of IL-12, IL-2, IL-18, or IL-4 at inflammatory 
      sites may differentially modulate the NK-cell interaction with DCs, leading to 
      different outcomes in naive T-cell polarization.
FAU - Agaugue, Sophie
AU  - Agaugue S
AD  - Dipartimento di Medicina Sperimentale, Universita degli Studi di Genova, Italy.
FAU - Marcenaro, Emanuela
AU  - Marcenaro E
FAU - Ferranti, Bruna
AU  - Ferranti B
FAU - Moretta, Lorenzo
AU  - Moretta L
FAU - Moretta, Alessandro
AU  - Moretta A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080625
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (IL2 protein, human)
RN  - 0 (IL4 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukins)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Cell Communication
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-12/pharmacology
MH  - Interleukin-18/pharmacology
MH  - Interleukin-2/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Interleukins/metabolism/*pharmacology
MH  - Killer Cells, Natural/*drug effects/*immunology
MH  - Monocytes/cytology/immunology
MH  - T-Lymphocytes/cytology/*immunology
MH  - Th1 Cells/cytology/immunology
EDAT- 2008/06/27 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/06/27 09:00
PHST- 2008/06/27 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/06/27 09:00 [entrez]
AID - S0006-4971(20)49611-9 [pii]
AID - 10.1182/blood-2008-02-135871 [doi]
PST - ppublish
SO  - Blood. 2008 Sep 1;112(5):1776-83. doi: 10.1182/blood-2008-02-135871. Epub 2008 
      Jun 25.