PMID- 18579868
OWN - NLM
STAT- MEDLINE
DCOM- 20080812
LR  - 20211020
IS  - 1469-9001 (Electronic)
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 14
IP  - 8
DP  - 2008 Aug
TI  - Fragile X mental retardation protein interactions with the microtubule associated 
      protein 1B RNA.
PG  - 1644-55
LID - 10.1261/rna.1100708 [doi]
AB  - Fragile X mental retardation syndrome, the most common form of inherited mental 
      retardation, is caused by the absence of the fragile X mental retardation protein 
      (FMRP). FMRP has been shown to use its arginine-glycine-glycine (RGG) box to bind 
      to a subset of RNA targets that form a G quadruplex structure. We performed a 
      detailed analysis of the interactions between the FMRP RGG box and the 
      microtubule associated protein 1B (MAP1B) mRNA, a relevant in vivo FMRP target. 
      We show that MAP1B RNA forms an intramolecular G quadruplex structure, which is 
      bound with high affinity and specificity by the FMRP RGG box. We determined that 
      hydrophobic interactions are important in the FMRP RGG box-MAP1B RNA association, 
      with minor contributions from electrostatic interactions. Our findings that at 
      low protein:RNA ratios the RNA G quadruplex structure is slightly stabilized, 
      whereas at high ratios is unfolded, suggest a mechanism by which the FMRP 
      concentration variation in response to a neurotransmitter stimulation event could 
      act as a regulatory switch for the protein function, from translation repressor 
      to translation activator.
FAU - Menon, Lakshmi
AU  - Menon L
AD  - Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, 
      Pennsylvania 15282, USA.
FAU - Mader, Samantha Ann
AU  - Mader SA
FAU - Mihailescu, Mihaela-Rita
AU  - Mihailescu MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080625
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (microtubule-associated protein 1B)
RN  - 139135-51-6 (Fragile X Mental Retardation Protein)
SB  - IM
MH  - Animals
MH  - Fragile X Mental Retardation Protein/chemistry/*metabolism
MH  - Humans
MH  - Microtubule-Associated Proteins/*genetics/*metabolism
MH  - Protein Biosynthesis
MH  - Protein Structure, Tertiary
MH  - RNA, Messenger/chemistry/metabolism
MH  - Thermodynamics
PMC - PMC2491469
EDAT- 2008/06/27 09:00
MHDA- 2008/08/13 09:00
PMCR- 2009/02/01
CRDT- 2008/06/27 09:00
PHST- 2008/06/27 09:00 [pubmed]
PHST- 2008/08/13 09:00 [medline]
PHST- 2008/06/27 09:00 [entrez]
PHST- 2009/02/01 00:00 [pmc-release]
AID - rna.1100708 [pii]
AID - RA [pii]
AID - 10.1261/rna.1100708 [doi]
PST - ppublish
SO  - RNA. 2008 Aug;14(8):1644-55. doi: 10.1261/rna.1100708. Epub 2008 Jun 25.