PMID- 18586294
OWN - NLM
STAT- MEDLINE
DCOM- 20080923
LR  - 20211203
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 378
IP  - 2
DP  - 2008 Sep 1
TI  - A human TRIM5alpha B30.2/SPRY domain mutant gains the ability to restrict and 
      prematurely uncoat B-tropic murine leukemia virus.
PG  - 233-42
LID - 10.1016/j.virol.2008.05.008 [doi]
AB  - Human TRIM5alpha restricts N-tropic murine leukemia virus (N-MLV) but not 
      B-tropic MLV (B-MLV) infection. Here we study B30.2/SPRY domain mutants of human 
      TRIM5alpha that acquire the ability to inhibit B-MLV infection prior to reverse 
      transcription without losing the ability to restrict N-MLV infection. Remarkably, 
      these mutants gain the ability to decrease the amount of particulate B-MLV 
      capsids in the cytosol of infected cells. In addition, these mutants gain the 
      ability to restrict SIV(mac) and HIV-2 infection. B-MLV and SIV(mac) infections 
      were blocked by the mutant TRIM5alpha proteins prior to reverse transcription. 
      Thus, the range of retroviruses restricted by human TRIM5alpha can be increased 
      by changes in the B30.2/SPRY domain, which also result in the ability to cause 
      premature uncoating of the restricted retroviral capsid.
FAU - Diaz-Griffero, Felipe
AU  - Diaz-Griffero F
AD  - Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 
      Department of Pathology, Division of AIDS, Harvard Medical School, Boston, MA 
      02115, USA.
FAU - Perron, Michel
AU  - Perron M
FAU - McGee-Estrada, Kathleen
AU  - McGee-Estrada K
FAU - Hanna, Robert
AU  - Hanna R
FAU - Maillard, Pierre V
AU  - Maillard PV
FAU - Trono, Didier
AU  - Trono D
FAU - Sodroski, Joseph
AU  - Sodroski J
LA  - eng
GR  - R01 AI063987/AI/NIAID NIH HHS/United States
GR  - R01 AI063987-04/AI/NIAID NIH HHS/United States
GR  - AI60354/AI/NIAID NIH HHS/United States
GR  - AI063987/AI/NIAID NIH HHS/United States
GR  - R21 AI076094/AI/NIAID NIH HHS/United States
GR  - R21 AI076094-01/AI/NIAID NIH HHS/United States
GR  - P30 AI060354/AI/NIAID NIH HHS/United States
GR  - AI076094/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080630
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antiviral Restriction Factors)
RN  - 0 (Carrier Proteins)
RN  - 0 (Tripartite Motif Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.3.2.27 (TRIM5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Antiviral Restriction Factors
MH  - Capsid/metabolism
MH  - Carrier Proteins/genetics/*physiology
MH  - Cell Line
MH  - Cytoplasm/virology
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins/analysis/genetics
MH  - HIV-2/immunology
MH  - Humans
MH  - Leukemia Virus, Murine/*immunology/physiology
MH  - *Mutation, Missense
MH  - Protein Structure, Tertiary
MH  - Simian Immunodeficiency Virus/immunology
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases
PMC - PMC2597210
MID - NIHMS67179
EDAT- 2008/07/01 09:00
MHDA- 2008/09/24 09:00
PMCR- 2009/09/01
CRDT- 2008/07/01 09:00
PHST- 2008/01/10 00:00 [received]
PHST- 2008/01/31 00:00 [revised]
PHST- 2008/05/05 00:00 [accepted]
PHST- 2008/07/01 09:00 [pubmed]
PHST- 2008/09/24 09:00 [medline]
PHST- 2008/07/01 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - S0042-6822(08)00310-3 [pii]
AID - 10.1016/j.virol.2008.05.008 [doi]
PST - ppublish
SO  - Virology. 2008 Sep 1;378(2):233-42. doi: 10.1016/j.virol.2008.05.008. Epub 2008 
      Jun 30.