PMID- 18586353
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20211124
IS  - 1558-1497 (Electronic)
IS  - 0197-4580 (Linking)
VI  - 31
IP  - 4
DP  - 2010 Apr
TI  - Astrocyte phenotype in relation to Alzheimer-type pathology in the ageing brain.
PG  - 578-90
LID - 10.1016/j.neurobiolaging.2008.05.015 [doi]
AB  - Astrocyte pathology occurs in association with Alzheimer's disease (AD) and in 
      brain ageing, but is poorly characterised. We sought to define the detailed 
      cellular pathology of astrocytes, the extent of population variation and the 
      relationship to Alzheimer-type changes in a population-based cohort. Three 
      staining patterns were associated with GFAP and excitatory amino acid transporter 
      2 (EAAT2): minimal, moderate or extensive immunoreactivity. GFAP and EAAT2 
      expression were inversely related (p=0.015), with trends to increased expression 
      of GFAP (p=0.019) and decreased expression of EAAT2 (p=ns) with increasing Braak 
      stage. GFAP and EAAT2 correlated incompletely with beta-amyloid and tau 
      immunoreactivity. However, gliosis increased with increasing burden of neuritic 
      (p=0.011), but not diffuse (p=ns), plaques. Double-staining revealed distinct 
      subsets of astrocytes; GFAP(+)EAAT(-), GFAP(-)EAAT(+), or GFAP(+)EAAT(+). In 
      contrast to the variation in GFAP and EAAT2, levels of EAAT1 and S100B showed 
      consistent staining patterns. Alzheimer-type pathology only partially explains 
      the variation in gliosis and astrocyte functional markers, suggesting that other 
      factors contribute to the population variance in astrocyte pathology.
CI  - Copyright (c) 2008 Elsevier Inc. All rights reserved.
FAU - Simpson, J E
AU  - Simpson JE
AD  - Academic Unit of Pathology, University of Sheffield Medical School, Beech Hill 
      Road, Sheffield S10 2RX, UK.
FAU - Ince, P G
AU  - Ince PG
FAU - Lace, G
AU  - Lace G
FAU - Forster, G
AU  - Forster G
FAU - Shaw, P J
AU  - Shaw PJ
FAU - Matthews, F
AU  - Matthews F
FAU - Savva, G
AU  - Savva G
FAU - Brayne, C
AU  - Brayne C
FAU - Wharton, S B
AU  - Wharton SB
CN  - MRC Cognitive Function and Ageing Neuropathology Study Group
LA  - eng
GR  - G0300126/MRC_/Medical Research Council/United Kingdom
GR  - G9901400/MRC_/Medical Research Council/United Kingdom
GR  - MC_U105292687/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20080630
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Biomarkers)
RN  - 0 (Excitatory Amino Acid Transporter 1)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (S100 Calcium Binding Protein beta Subunit)
RN  - 0 (S100 Proteins)
RN  - 0 (S100B protein, human)
RN  - 0 (tau Proteins)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/metabolism/*pathology/physiopathology
MH  - Amyloid beta-Peptides/analysis/metabolism
MH  - Astrocytes/metabolism/*pathology
MH  - Biomarkers/analysis/metabolism
MH  - Brain/metabolism/*pathology/physiopathology
MH  - Cohort Studies
MH  - Excitatory Amino Acid Transporter 1/analysis/metabolism
MH  - Excitatory Amino Acid Transporter 2/analysis/metabolism
MH  - Female
MH  - Glial Fibrillary Acidic Protein/analysis/metabolism
MH  - Gliosis/metabolism/*pathology/physiopathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Longitudinal Studies
MH  - Male
MH  - Nerve Growth Factors/analysis/metabolism
MH  - Nerve Tissue Proteins/analysis/metabolism
MH  - Phenotype
MH  - Plaque, Amyloid/metabolism/pathology
MH  - Predictive Value of Tests
MH  - Prospective Studies
MH  - S100 Calcium Binding Protein beta Subunit
MH  - S100 Proteins/analysis/metabolism
MH  - Severity of Illness Index
MH  - tau Proteins/analysis/metabolism
EDAT- 2008/07/01 09:00
MHDA- 2010/06/09 06:00
CRDT- 2008/07/01 09:00
PHST- 2007/12/07 00:00 [received]
PHST- 2008/02/29 00:00 [revised]
PHST- 2008/05/18 00:00 [accepted]
PHST- 2008/07/01 09:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
PHST- 2008/07/01 09:00 [entrez]
AID - S0197-4580(08)00170-X [pii]
AID - 10.1016/j.neurobiolaging.2008.05.015 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2010 Apr;31(4):578-90. doi: 
      10.1016/j.neurobiolaging.2008.05.015. Epub 2008 Jun 30.