PMID- 18586975
OWN - NLM
STAT- MEDLINE
DCOM- 20080919
LR  - 20220216
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 74
IP  - 16
DP  - 2008 Aug
TI  - Quantitative microbial community analysis of three different sulfidic mine 
      tailing dumps generating acid mine drainage.
PG  - 5211-9
LID - 10.1128/AEM.00649-08 [doi]
AB  - The microbial communities of three different sulfidic and acidic mine waste 
      tailing dumps located in Botswana, Germany, and Sweden were quantitatively 
      analyzed using quantitative real-time PCR (Q-PCR), fluorescence in situ 
      hybridization (FISH), catalyzed reporter deposition-FISH (CARD-FISH), Sybr green 
      II direct counting, and the most probable number (MPN) cultivation technique. 
      Depth profiles of cell numbers showed that the compositions of the microbial 
      communities are greatly different at the three sites and also strongly varied 
      between zones of oxidized and unoxidized tailings. Maximum cell numbers of up to 
      10(9) cells g(-1) dry weight were determined in the pyrite or pyrrhotite 
      oxidation zones, whereas cell numbers in unoxidized tailings were significantly 
      lower. Bacteria dominated over Archaea and Eukarya at all tailing sites. The 
      acidophilic Fe(II)- and/or sulfur-oxidizing Acidithiobacillus spp. dominated over 
      the acidophilic Fe(II)-oxidizing Leptospirillum spp. among the Bacteria at two 
      sites. The two genera were equally abundant at the third site. The acidophilic 
      Fe(II)- and sulfur-oxidizing Sulfobacillus spp. were generally less abundant. The 
      acidophilic Fe(III)-reducing Acidiphilium spp. could be found at only one site. 
      The neutrophilic Fe(III)-reducing Geobacteraceae as well as the dsrA gene of 
      sulfate reducers were quantifiable at all three sites. FISH analysis provided 
      reliable data only for tailing zones with high microbial activity, whereas 
      CARD-FISH, Q-PCR, Sybr green II staining, and MPN were suitable methods for a 
      quantitative microbial community analysis of tailings in general.
FAU - Kock, Dagmar
AU  - Kock D
AD  - Federal Institute for Geosciences and Natural Resources (BGR), Stilleweg 2, 30655 
      Hannover, Germany.
FAU - Schippers, Axel
AU  - Schippers A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080627
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Acids)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Industrial Waste)
RN  - 0 (Metals, Heavy)
RN  - 0 (Organic Chemicals)
RN  - 0 (SYBR Green II)
SB  - IM
MH  - Acids/metabolism
MH  - Archaea/growth & development
MH  - Bacteria/*growth & development/metabolism
MH  - Botswana
MH  - Colony Count, Microbial
MH  - DNA, Bacterial/analysis
MH  - Germany
MH  - In Situ Hybridization, Fluorescence
MH  - *Industrial Waste
MH  - Metals, Heavy/metabolism
MH  - *Mining
MH  - Organic Chemicals
MH  - Oxidation-Reduction
MH  - Polymerase Chain Reaction
MH  - Soil Microbiology
MH  - Sweden
MH  - Water Microbiology
PMC - PMC2519280
EDAT- 2008/07/01 09:00
MHDA- 2008/09/20 09:00
PMCR- 2008/12/27
CRDT- 2008/07/01 09:00
PHST- 2008/07/01 09:00 [pubmed]
PHST- 2008/09/20 09:00 [medline]
PHST- 2008/07/01 09:00 [entrez]
PHST- 2008/12/27 00:00 [pmc-release]
AID - AEM.00649-08 [pii]
AID - 0649-08 [pii]
AID - 10.1128/AEM.00649-08 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2008 Aug;74(16):5211-9. doi: 10.1128/AEM.00649-08. Epub 
      2008 Jun 27.