PMID- 18587175
OWN - NLM
STAT- MEDLINE
DCOM- 20081126
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 57
IP  - 12
DP  - 2008
TI  - Analysis of nitrifying bacterial communities in aerobic biofilm reactors with 
      different DO conditions using molecular techniques.
PG  - 1889-99
LID - 10.2166/wst.2008.622 [doi]
AB  - In order to assess the relationship between the dissolved oxygen (DO) 
      concentration and the characteristics of nitrifying bacterial communities in an 
      aerobic biofilm reactor, molecular techniques including denaturing gradient gel 
      electrophoresis (DGGE)/cloning based on PCR targeting 16S rRNA and the amoA gene 
      and fluorescence in situ hybridisation (FISH) were conducted. The D-1, D-2, D-3 
      and D-4 reactors with different DO concentrations (1, 3, 5 and 7 mg/L, 
      respectively) were set up in the thermostat and acclimated. The optimal DO 
      concentration with stable nitrification efficiency was above 5.0 mg/L. As was 
      shown by the results of DGGE and cloning, the community of ammonia-oxidising 
      bacteria (AOB) and the ratio of Nitrosomonas sp. changed only slightly despite 
      their differing nitrification efficiencies. The results of FISH indicated that 
      higher DO concentrations resulted in an increase in AOB and nitrite-oxidising 
      bacteria (NOB), and a reduction in heterotrophic microorganisms. The 
      INT-dehydrogenase activity (DHA) test demonstrated that the activity of AOB 
      decreased with reductions in the DO concentration. This means that the DO 
      concentration does not influence the community of AOB, but rather the activity of 
      AOB. In the relationship between the attached biomass and the nitrification 
      efficiency, only the active biomass affected the nitrification efficiencies.
CI  - IWA Publishing 2008.
FAU - Park, J J
AU  - Park JJ
AD  - School of Civil and Environmental Engineering, Pusan National University, 
      609-735, Busan, Korea.
FAU - Byun, I G
AU  - Byun IG
FAU - Yu, J C
AU  - Yu JC
FAU - Park, S R
AU  - Park SR
FAU - Ju, D J
AU  - Ju DJ
FAU - Hur, S H
AU  - Hur SH
FAU - Park, T J
AU  - Park TJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Nitrites)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aerobiosis
MH  - Ammonia/metabolism
MH  - Bacteria, Aerobic/genetics/metabolism/*physiology
MH  - *Biofilms
MH  - Bioreactors/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrites/metabolism
MH  - Nitrosomonas/genetics/metabolism/physiology
MH  - Oxidation-Reduction
MH  - Oxygen/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2008/07/01 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/07/01 09:00
PHST- 2008/07/01 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/07/01 09:00 [entrez]
AID - 10.2166/wst.2008.622 [doi]
PST - ppublish
SO  - Water Sci Technol. 2008;57(12):1889-99. doi: 10.2166/wst.2008.622.