PMID- 18588555
OWN - NLM
STAT- MEDLINE
DCOM- 20080730
LR  - 20220410
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 63
IP  - 7
DP  - 2008 Jul
TI  - Evaluation by double-blind placebo-controlled oral challenge of the clinical 
      relevance of IgE antibodies against plant glycans.
PG  - 891-6
LID - 10.1111/j.1398-9995.2008.01703.x [doi]
AB  - BACKGROUND: The clinical relevance of immunoglobulin E (IgE) to plant glycans is 
      a longstanding debate. We sought to evaluate their clinical reactivity using the 
      human glycoprotein lactoferrin expressed in rice. METHODS: Allergic patients with 
      IgE antibodies against plant glycans were analyzed for the presence of IgE 
      against rice-produced lactoferrin. The potency of IgE to induce mediator release 
      was assessed by basophil histamine release and skin prick tests (SPTs). Clinical 
      relevance was evaluated by double-blind placebo-controlled oral challenge 
      (DBPCOC). RESULTS: Twenty-four of 29 sera (82.7%) with IgE antibodies against 
      plant glycans demonstrated IgE binding to transgenic lactoferrin. In three of 
      five cases transgenic lactoferrin induced histamine release. Compared to a 
      control major grass pollen allergen lactoferrin concentrations needed for 
      biological activity of IgE were 5-6 orders of magnitude higher. Skin prick test 
      and DBPCOC were negative in five patients with potential clinical reactivity that 
      volunteered to undergo these in vivo challenges. CONCLUSIONS: Poor or no 
      biological activity and lack of clinical relevance of IgE-binding plant glycans 
      (five out of five) was demonstrated using human lactoferrin expressed in rice as 
      a model.
FAU - Mari, A
AU  - Mari A
AD  - Center for Clinical and Experimental Allergology, IDI-IRCCS, Rome, Italy.
FAU - Ooievaar-de Heer, P
AU  - Ooievaar-de Heer P
FAU - Scala, E
AU  - Scala E
FAU - Giani, M
AU  - Giani M
FAU - Pirrotta, L
AU  - Pirrotta L
FAU - Zuidmeer, L
AU  - Zuidmeer L
FAU - Bethell, D
AU  - Bethell D
FAU - van Ree, R
AU  - van Ree R
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Carrier Proteins)
RN  - 0 (LTF protein, human)
RN  - 0 (Plant Proteins)
RN  - 0 (Polysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.21.- (Lactoferrin)
SB  - IM
MH  - Adolescent
MH  - Allergens/*immunology
MH  - Basophil Degranulation Test
MH  - Carrier Proteins/genetics/immunology
MH  - Child
MH  - Double-Blind Method
MH  - Female
MH  - Histamine Release
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/blood/*immunology
MH  - Lactoferrin
MH  - Male
MH  - Middle Aged
MH  - Oryza/genetics
MH  - Phleum/immunology
MH  - Plant Proteins/immunology
MH  - Plants, Genetically Modified/genetics
MH  - Pollen/immunology
MH  - Polysaccharides/*immunology
MH  - Radioallergosorbent Test
MH  - Recombinant Proteins/genetics/immunology
MH  - Skin Tests
EDAT- 2008/07/01 09:00
MHDA- 2008/07/31 09:00
CRDT- 2008/07/01 09:00
PHST- 2008/07/01 09:00 [pubmed]
PHST- 2008/07/31 09:00 [medline]
PHST- 2008/07/01 09:00 [entrez]
AID - ALL1703 [pii]
AID - 10.1111/j.1398-9995.2008.01703.x [doi]
PST - ppublish
SO  - Allergy. 2008 Jul;63(7):891-6. doi: 10.1111/j.1398-9995.2008.01703.x.