PMID- 18588880
OWN - NLM
STAT- MEDLINE
DCOM- 20080915
LR  - 20161124
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 582
IP  - 17
DP  - 2008 Jul 23
TI  - Sirtuin-mediated deacetylation pathway stabilizes Werner syndrome protein.
PG  - 2479-83
LID - 10.1016/j.febslet.2008.06.031 [doi]
AB  - Caloric restriction (CR) is known to promote longevity in various species. 
      Sirtuin-mediated deacetylation has been shown to be related to the promotion of 
      longevity in some species. Here, we show that CR of rats led to an increase in 
      the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In 
      addition, CR simultaneously increased the level of SIRT1, a mammalian sirtuin. In 
      HEK293T cells, sirtuin inhibitors decreased the WRN level, and this effect was 
      suppressed by proteasomal inhibitors. Furthermore, we found a decrease in the WRN 
      level in Sirt1-deficient mice. These results indicate that sirtuin-mediated 
      deacetylation stabilizes WRN. STRUCTURED SUMMARY:
FAU - Kahyo, Tomoaki
AU  - Kahyo T
AD  - Molecular Gerontology Research Group, Mitsubishi Kagaku Institute of Life 
      Sciences, Minamiooya, Machida, Tokyo, Japan.
FAU - Mostoslavsky, Raul
AU  - Mostoslavsky R
FAU - Goto, Makoto
AU  - Goto M
FAU - Setou, Mitsutoshi
AU  - Setou M
LA  - eng
PT  - Journal Article
DEP - 20080625
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Enzyme Inhibitors)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - EC 3.4.99.- (ATP dependent 26S protease)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - EC 3.5.1.- (Sirtuins)
RN  - EC 3.6.4.12 (RecQ Helicases)
RN  - EC 3.6.4.12 (WRN protein, human)
RN  - EC 3.6.4.12 (Werner Syndrome Helicase)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - *Caloric Restriction
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme Stability
MH  - Exodeoxyribonucleases/*metabolism
MH  - Humans
MH  - Liver/enzymology
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Proteasome Endopeptidase Complex/metabolism
MH  - Rats
MH  - RecQ Helicases/*metabolism
MH  - Sirtuin 1
MH  - Sirtuins/antagonists & inhibitors/genetics/*metabolism
MH  - Werner Syndrome Helicase
EDAT- 2008/07/01 09:00
MHDA- 2008/09/16 09:00
CRDT- 2008/07/01 09:00
PHST- 2008/04/14 00:00 [received]
PHST- 2008/06/09 00:00 [revised]
PHST- 2008/06/10 00:00 [accepted]
PHST- 2008/07/01 09:00 [pubmed]
PHST- 2008/09/16 09:00 [medline]
PHST- 2008/07/01 09:00 [entrez]
AID - S0014-5793(08)00542-5 [pii]
AID - 10.1016/j.febslet.2008.06.031 [doi]
PST - ppublish
SO  - FEBS Lett. 2008 Jul 23;582(17):2479-83. doi: 10.1016/j.febslet.2008.06.031. Epub 
      2008 Jun 25.