PMID- 18590926
OWN - NLM
STAT- MEDLINE
DCOM- 20090210
LR  - 20220409
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 73
IP  - 1
DP  - 2008 Aug
TI  - Laccase mediated biodegradation of 2,4-dichlorophenol using response surface 
      methodology.
PG  - 81-5
LID - 10.1016/j.chemosphere.2008.05.005 [doi]
AB  - The effects of different environmental parameters, i.e., pH, temperature, time 
      and enzyme concentration on the biodegradation of 2,4-dichlorophenol (2,4-DCP) in 
      aqueous phase was evaluated with laccase from Pleurotus sp. using response 
      surface methodology (RSM) in the present investigation. The Box-Behnken design of 
      experiments was used to construct second order response surfaces with the 
      investigated parameters. It was observed that the maximum degradation efficiency 
      of approximately 98% was achieved at pH 6, temperature of 40 degrees C, time 9h 
      and an enzyme concentration of 8 IU ml(-1). The adequacy of the model was 
      confirmed by the coefficient of multiple regression, R(2) and adjusted R(2) which 
      were adjudged to be 87.9% and 73.6%, respectively indicating a reasonably good 
      model for practical implementation. Despite the fact that many successful 
      attempts have been taken in the past for biodegradation of 2,4-DCP using whole 
      cells, the present study emphasizes the fastest biodegradation of 2,4-DCP, a 
      potent xenobiotic compound.
FAU - Bhattacharya, S S
AU  - Bhattacharya SS
AD  - Microbial Biotechnology and Downstream Processing Laboratory, Indian Institute of 
      Technology, Kharagpur 721302, India.
FAU - Banerjee, R
AU  - Banerjee R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080630
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Chlorophenols)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Xenobiotics)
RN  - EC 1.10.3.2 (Laccase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
EIN - Chemosphere. 2009 Jan;74(3):479
MH  - Biodegradation, Environmental
MH  - Chlorophenols/analysis/*metabolism
MH  - Environmental Pollutants/analysis/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Laccase/*metabolism
MH  - Models, Statistical
MH  - Pleurotus/metabolism
MH  - Temperature
MH  - Time Factors
MH  - Xenobiotics/analysis
EDAT- 2008/07/02 09:00
MHDA- 2009/02/12 09:00
CRDT- 2008/07/02 09:00
PHST- 2008/01/13 00:00 [received]
PHST- 2008/04/26 00:00 [revised]
PHST- 2008/05/05 00:00 [accepted]
PHST- 2008/07/02 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
PHST- 2008/07/02 09:00 [entrez]
AID - S0045-6535(08)00624-3 [pii]
AID - 10.1016/j.chemosphere.2008.05.005 [doi]
PST - ppublish
SO  - Chemosphere. 2008 Aug;73(1):81-5. doi: 10.1016/j.chemosphere.2008.05.005. Epub 
      2008 Jun 30.