PMID- 18592413
OWN - NLM
STAT- MEDLINE
DCOM- 20090217
LR  - 20221207
IS  - 0895-8696 (Print)
IS  - 0895-8696 (Linking)
VI  - 36
IP  - 1-3
DP  - 2008 Nov
TI  - Involvement of pituitary adenylate cyclase activating polypeptide (PACAP) and its 
      receptors in the mechanism of antidepressant action.
PG  - 330-8
LID - 10.1007/s12031-008-9116-0 [doi]
AB  - Recent studies have suggested antidepressant involvement in synaptic plasticity, 
      possibly mediated by neurotrophins and neuropeptides. Pituitary adenylate cyclase 
      activating polypeptide (PACAP) is a neuropeptide and neuromodulator. Since its 
      discovery, PACAP has been extensively investigated with regard to its 
      neurotrophic properties including regulation of brain-derived neurotrophic factor 
      (BDNF) expression, a neurotrophin postulated to be involved in the mechanism of 
      antidepressant action and etiology of affective disorders. Using real-time 
      polymerase chain reaction (PCR) technique, we demonstrate in this paper a robust 
      upregulation of BDNF messenger RNA (mRNA) expression in rat primary cortical 
      neurons following a 6-hour incubation with PACAP, and subsequently elevated BDNF 
      expression after prolonged treatment. Additional experiments were conducted to 
      evaluate the effects of antidepressants on the expression of PACAP, its receptors 
      and BDNF. In rat hippocampal neurons, prolonged (72-hour) treatment with 
      selective serotonin reuptake inhibitors paroxetine and citalopram significantly 
      up-regulated BDNF and PACAP expression and down-regulated PACAP receptor (PAC1 
      and VPAC2) expression; the tricyclic antidepressant imipramine had an opposite 
      effect. These alterations in BDNF expression correlated negatively with PAC1 and 
      VPAC2 expression, and positively with PACAP mRNA levels. Thus, our findings 
      suggest the possible involvement of PACAP signaling in the neuronal plasticity 
      induced by antidepressant treatment.
FAU - Reichenstein, Michal
AU  - Reichenstein M
AD  - Department of Molecular Biology, Ariel University Center of Samaria, Ariel, 
      40700, Israel.
FAU - Rehavi, Moshe
AU  - Rehavi M
FAU - Pinhasov, Albert
AU  - Pinhasov A
LA  - eng
PT  - Journal Article
DEP - 20080701
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Adrenergic Uptake Inhibitors)
RN  - 0 (Antidepressive Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 0DHU5B8D6V (Citalopram)
RN  - 41VRH5220H (Paroxetine)
RN  - OGG85SX4E4 (Imipramine)
SB  - IM
MH  - Adrenergic Uptake Inhibitors/pharmacology
MH  - Animals
MH  - Antidepressive Agents/metabolism/*pharmacology
MH  - Brain/cytology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Citalopram/pharmacology
MH  - Imipramine/pharmacology
MH  - Neurons/cytology/drug effects/metabolism
MH  - Paroxetine/pharmacology
MH  - Pituitary Adenylate Cyclase-Activating Polypeptide/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Pituitary Adenylate Cyclase-Activating 
      Polypeptide/genetics/*metabolism
MH  - Selective Serotonin Reuptake Inhibitors/pharmacology
MH  - Signal Transduction/physiology
EDAT- 2008/07/02 09:00
MHDA- 2009/02/20 09:00
CRDT- 2008/07/02 09:00
PHST- 2008/04/10 00:00 [received]
PHST- 2008/05/29 00:00 [accepted]
PHST- 2008/07/02 09:00 [pubmed]
PHST- 2009/02/20 09:00 [medline]
PHST- 2008/07/02 09:00 [entrez]
AID - 10.1007/s12031-008-9116-0 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2008 Nov;36(1-3):330-8. doi: 10.1007/s12031-008-9116-0. Epub 2008 
      Jul 1.