PMID- 18593646
OWN - NLM
STAT- MEDLINE
DCOM- 20081028
LR  - 20191210
IS  - 1873-488X (Electronic)
IS  - 1056-8719 (Linking)
VI  - 58
IP  - 1
DP  - 2008 Jul-Aug
TI  - Interlaboratory validation of the modified murine local lymph node assay based on 
      adenosine triphosphate measurement.
PG  - 11-26
LID - 10.1016/j.vascn.2008.05.001 [doi]
AB  - INTRODUCTION: The murine local lymph node assay (LLNA) is a well-established 
      alternative to the guinea pig maximization test (GPMT) or Buehler test (BT) for 
      the assessment of the skin sensitizing ability of drugs and chemicals. Daicel 
      Chemical Industries Ltd. has developed a modified LLNA based on the adenosine 
      triphosphate (ATP) content (LLNA-DA). We conducted 2 interlaboratory validation 
      studies to evaluate the reliability and relevance of LLNA-DA. METHODS: The 
      experiment involved 17 laboratories, wherein 14 chemicals were examined under 
      blinded conditions. In the first study, 3 chemicals were examined in 10 
      laboratories and the remaining 9 were examined in 3 laboratories. In the second 
      study, 1 chemical was examined in 7 laboratories and the remaining 4 chemicals 
      were examined in 4 laboratories. The data were expressed as the ATP content for 
      each chemical-treated group, and the stimulation index (SI) for each 
      chemical-treated group was determined as the increase in the ATP content relative 
      to the concurrent vehicle control group. An SI of 3 was set as the cut-off value 
      for exhibiting skin sensitization activity. RESULTS: The results of the first 
      study obtained in the experiments conducted for the 3 chemicals that were 
      examined in all the 10 laboratories and for 5 of the remaining 9 chemicals were 
      sufficiently consistent with small variations in their SI values. The 
      sensitivity, specificity, and accuracy of LLNA-DA against those of GPMT/BT were 
      7/8 (87.5%), 3/3 (100%), and 10/11 (90.9%), respectively. In the second study, 
      all the 5 chemicals studied demonstrated acceptably small interlaboratory 
      variations. DISCUSSION: In the first study, a large variation was observed for 2 
      chemicals; in the second study, this variation was small. It was attributed to 
      the application of dimethylsulfoxide as the solvent for the metallic salts. In 
      conclusion, these 2 studies provide good evidence for the reliability of the 
      LLNA-DA.
FAU - Omori, Takashi
AU  - Omori T
AD  - Kyoto University School of Public Health, Japan. omori@pbh.med.kyoto-u.ac.jp
FAU - Idehara, Kenji
AU  - Idehara K
FAU - Kojima, Hajime
AU  - Kojima H
FAU - Sozu, Takashi
AU  - Sozu T
FAU - Arima, Kazunori
AU  - Arima K
FAU - Goto, Hirohiko
AU  - Goto H
FAU - Hanada, Tomohiko
AU  - Hanada T
FAU - Ikarashi, Yoshiaki
AU  - Ikarashi Y
FAU - Inoda, Taketo
AU  - Inoda T
FAU - Kanazawa, Yukiko
AU  - Kanazawa Y
FAU - Kosaka, Tadashi
AU  - Kosaka T
FAU - Maki, Eiji
AU  - Maki E
FAU - Morimoto, Takashi
AU  - Morimoto T
FAU - Shinoda, Shinsuke
AU  - Shinoda S
FAU - Shinoda, Naoki
AU  - Shinoda N
FAU - Takeyoshi, Masahiro
AU  - Takeyoshi M
FAU - Tanaka, Masashi
AU  - Tanaka M
FAU - Uratani, Mamoru
AU  - Uratani M
FAU - Usami, Masahito
AU  - Usami M
FAU - Yamanaka, Atsushi
AU  - Yamanaka A
FAU - Yoneda, Tomofumi
AU  - Yoneda T
FAU - Yoshimura, Isao
AU  - Yoshimura I
FAU - Yuasa, Atsuko
AU  - Yuasa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20080521
PL  - United States
TA  - J Pharmacol Toxicol Methods
JT  - Journal of pharmacological and toxicological methods
JID - 9206091
RN  - 0 (Irritants)
RN  - 0 (Solvents)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Dermatitis, Allergic Contact/diagnosis/*etiology
MH  - Female
MH  - Irritants/*toxicity
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Reproducibility of Results
MH  - Solvents/chemistry
MH  - Toxicity Tests/methods
EDAT- 2008/07/03 09:00
MHDA- 2008/10/29 09:00
CRDT- 2008/07/03 09:00
PHST- 2008/03/27 00:00 [received]
PHST- 2008/05/07 00:00 [accepted]
PHST- 2008/07/03 09:00 [pubmed]
PHST- 2008/10/29 09:00 [medline]
PHST- 2008/07/03 09:00 [entrez]
AID - S1056-8719(08)00028-2 [pii]
AID - 10.1016/j.vascn.2008.05.001 [doi]
PST - ppublish
SO  - J Pharmacol Toxicol Methods. 2008 Jul-Aug;58(1):11-26. doi: 
      10.1016/j.vascn.2008.05.001. Epub 2008 May 21.