PMID- 18597000
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20080703
IS  - 0379-0355 (Print)
IS  - 0379-0355 (Linking)
VI  - 30
IP  - 3
DP  - 2008 Apr
TI  - Low-molecular weight molluscan glycosaminoglycan from bivalve Katelysia opima 
      (Gmelin).
PG  - 175-80
LID - 10.1358/mf.2008.30.3.1159654 [doi]
AB  - Heparin, the glycosaminoglycan (GAG), was isolated from mollusk Katelysia opima 
      (bivalve). The crude extract was partially purified by DEAE cellulose 
      fractionation using two different molar concentrations of saline solution. Among 
      the fractions F1, F2, F3 and F4, fraction F3 had highmolecular weight and better 
      activity. F3 was further processed to get low-molecular weight heparin (LMWH) by 
      nitrous acid depolymerization, and the total yield was 1.2 g/kg. The crude 
      unfractionated heparin (UFH), fractionated heparin and depolymerized heparin 
      samples were colorimetrically analyzed for the metachromatic shift using azure-A 
      dye, and all the samples were found to be metachromatically active. Both UFH and 
      LMWH showed an anticoagulant activity of 160 USP U/mg. Fractionated molluscan and 
      LMWH were found to have peaks similar to that of standard heparin when assessed 
      by the FTIR spectrum. Finally, molecular weight was determined by gradient 
      polyacrylamide gel electrophoresis for UFH, fractionated F3 and LMWH, which were 
      found to be 31,000, 30,500 and 6000 Da, respectively.
CI  - Copyright 2008 Prous Science, S.A.U. or its licensors. All rights reserved.
FAU - Vijayabaskar, P
AU  - Vijayabaskar P
AD  - Centre of Advanced Study in Marine Biology, Annamalai University, Tamilnadu, 
      India.
FAU - Balasubramanian, T
AU  - Balasubramanian T
FAU - Somasundaram, S T
AU  - Somasundaram ST
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Methods Find Exp Clin Pharmacol
JT  - Methods and findings in experimental and clinical pharmacology
JID - 7909595
RN  - 0 (Anticoagulants)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Anticoagulants/*chemical synthesis/chemistry/isolation & purification
MH  - *Bivalvia
MH  - Glycosaminoglycans/*isolation & purification
MH  - Heparin/*chemical synthesis/chemistry/isolation & purification
MH  - Heparin, Low-Molecular-Weight/*chemical synthesis/chemistry/isolation & 
      purification
MH  - India
MH  - *Shellfish
EDAT- 2008/07/04 09:00
MHDA- 2008/11/04 09:00
CRDT- 2008/07/04 09:00
PHST- 2008/07/04 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/07/04 09:00 [entrez]
AID - 1159654 [pii]
AID - 10.1358/mf.2008.30.3.1159654 [doi]
PST - ppublish
SO  - Methods Find Exp Clin Pharmacol. 2008 Apr;30(3):175-80. doi: 
      10.1358/mf.2008.30.3.1159654.